PDF(Pubmed)
Abstract：
UNASSIGNED: Traumatic optic neuropathy (TON) is the optic nerve injury secondary to brain trauma leading to visual impairment and vision loss. Current clinical visual function assessments often fail to detect TON due to slow disease progression and clinically silent lesions resulting in potentially delayed or missed treatment in patients with traumatic brain injury (TBI).
UNASSIGNED: Diffusion basis spectrum imaging (DBSI) is a novel imaging modality that can potentially fill this diagnostic gap. Twenty-two, 16-week-old, male mice were equally divided into a sham or TBI (induced by moderate Closed-Head Impact Model of Engineered Rotational Acceleration device) group. Briefly, mice were anesthetized with isoflurane (5% for 2.5 min followed by 2.5% maintenance during injury induction), had a helmet placed over the head, and were placed in a holder prior to a 2.1-joule impact. Serial visual acuity (VA) assessments, using the Virtual Optometry System, and DBSI scans were performed in both groups of mice. Immunohistochemistry (IHC) and histological analysis of optic nerves was also performed after in vivo MRI.
UNASSIGNED: VA of the TBI mice showed unilateral or bilateral impairment. DBSI of the optic nerves exhibited bilateral involvement. IHC results of the optic nerves revealed axonal loss, myelin injury, axonal injury, and increased cellularity in the optic nerves of the TBI mice. Increased DBSI axon volume, decreased DBSI λ||, and elevated DBSI restricted fraction correlated with decreased SMI-312, decreased SMI-31, and increased DAPI density, respectively, suggesting that DBSI can detect coexisting pathologies in the optic nerves of TBI mice.
UNASSIGNED: DBSI provides an imaging modality capable of detecting subclinical changes of indirect TON in TBI mice.
UNASSIGNED: Diffusion basis spectrum imaging (DBSI) is a novel imaging modality that can potentially fill this diagnostic gap. Twenty-two, 16-week-old, male mice were equally divided into a sham or TBI (induced by moderate Closed-Head Impact Model of Engineered Rotational Acceleration device) group. Briefly, mice were anesthetized with isoflurane (5% for 2.5 min followed by 2.5% maintenance during injury induction), had a helmet placed over the head, and were placed in a holder prior to a 2.1-joule impact. Serial visual acuity (VA) assessments, using the Virtual Optometry System, and DBSI scans were performed in both groups of mice. Immunohistochemistry (IHC) and histological analysis of optic nerves was also performed after in vivo MRI.
UNASSIGNED: VA of the TBI mice showed unilateral or bilateral impairment. DBSI of the optic nerves exhibited bilateral involvement. IHC results of the optic nerves revealed axonal loss, myelin injury, axonal injury, and increased cellularity in the optic nerves of the TBI mice. Increased DBSI axon volume, decreased DBSI λ||, and elevated DBSI restricted fraction correlated with decreased SMI-312, decreased SMI-31, and increased DAPI density, respectively, suggesting that DBSI can detect coexisting pathologies in the optic nerves of TBI mice.
UNASSIGNED: DBSI provides an imaging modality capable of detecting subclinical changes of indirect TON in TBI mice.
摘要：
■外伤性视神经病变（TON）是继发于脑外伤导致视力障碍和视力丧失的视神经损伤。当前的临床视觉功能评估通常由于疾病进展缓慢和临床上无症状的病变而无法检测到TON，从而导致患有创伤性脑损伤（TBI）的患者可能延迟或错过治疗。
■扩散基础光谱成像（DBSI）是一种新颖的成像模式，可以潜在地填补这一诊断空白。二十二,16周大,将雄性小鼠等分为假手术组或TBI(由工程化旋转加速装置的中度闭合头部冲击模型诱导)组。简而言之，小鼠用异氟烷麻醉（5%，2.5分钟，然后在损伤诱导期间维持2.5%），头上戴了头盔,并在2.1焦耳冲击之前放置在支架中。连续视力（VA）评估，使用虚拟验光系统，在两组小鼠中进行DBSI扫描。在体内MRI后还进行了视神经的免疫组织化学（IHC）和组织学分析。
■TBI小鼠的VA显示单侧或双侧损伤。视神经的DBSI表现为双侧受累。视神经的IHC结果显示轴突丢失，髓鞘损伤,轴突损伤,并增加了TBI小鼠视神经的细胞数量。增加DBSI轴突体积，降低DBSIλ||，和升高的DBSI限制分数与SMI-312降低，SMI-31降低和DAPI密度增加相关，分别,表明DBSI可以检测TBI小鼠视神经中共存的病理。
■DBSI提供了能够检测TBI小鼠中间接TON的亚临床变化的成像模式。
■扩散基础光谱成像（DBSI）是一种新颖的成像模式，可以潜在地填补这一诊断空白。二十二,16周大,将雄性小鼠等分为假手术组或TBI(由工程化旋转加速装置的中度闭合头部冲击模型诱导)组。简而言之，小鼠用异氟烷麻醉（5%，2.5分钟，然后在损伤诱导期间维持2.5%），头上戴了头盔,并在2.1焦耳冲击之前放置在支架中。连续视力（VA）评估，使用虚拟验光系统，在两组小鼠中进行DBSI扫描。在体内MRI后还进行了视神经的免疫组织化学（IHC）和组织学分析。
■TBI小鼠的VA显示单侧或双侧损伤。视神经的DBSI表现为双侧受累。视神经的IHC结果显示轴突丢失，髓鞘损伤,轴突损伤,并增加了TBI小鼠视神经的细胞数量。增加DBSI轴突体积，降低DBSIλ||，和升高的DBSI限制分数与SMI-312降低，SMI-31降低和DAPI密度增加相关，分别,表明DBSI可以检测TBI小鼠视神经中共存的病理。
■DBSI提供了能够检测TBI小鼠中间接TON的亚临床变化的成像模式。
