PDF(Pubmed)
Abstract：
The signal recognition particle (SRP) system delivers approximately 30% of the proteome to the endoplasmic reticulum (ER) membrane. SRP receptor alpha (SRα) binds to SRP for targeting nascent secreted proteins to the ER membrane in eukaryotic cells. In this study, the SRα homologous gene was identified in the sea cucumber, Apostichopus japonicus (AjSRα). AjSRα codes for 641 amino acids and has 54.94% identity with its mammalian homologs. Like mammalian SRα, it is expected to contain the SRP-alpha N domain, SRP54_N domain, and SRP54 domain. In addition, AjSRα is ubiquitously expressed in adult tissues and exhibits a sexually dimorphic expression pattern, with significantly higher expression in ovaries compared to testes. As a maternal factor, AjSRα can be continuously detected during embryonic development. Importantly, we first attempted to investigate its function by using lentiviral vectors for delivering SRα gene-specific shRNA, and we revealed that lentiviral vectors do not induce an upregulation of immune-related enzymes in sea cucumbers. However, compared to the dsRNA-based RNA interference (RNAi) method, lentivirus-mediated RNAi caused dynamic changes in gene expression at a later time. This study supplied the technical support for studying the functional mechanism of SRα in sea cucumbers.
摘要：
信号识别颗粒(SRP)系统将大约30%的蛋白质组递送至内质网(ER)膜。SRP受体α（SRα）与SRP结合，以将新生分泌蛋白靶向真核细胞中的ER膜。在这项研究中,在海参中鉴定了SRα同源基因，刺参（AjSRα）。AjSRα编码641个氨基酸，与其哺乳动物同源物具有54.94％的同一性。像哺乳动物SRα一样，它预计包含SRP-αN域，SRP54_N域，和SRP54域。此外,AjSRα在成人组织中普遍表达，并表现出性别二态表达模式，与睾丸相比，卵巢中的表达明显更高。作为母性因素,在胚胎发育过程中可以连续检测到AjSRα。重要的是,我们首先尝试通过使用慢病毒载体递送SRα基因特异性shRNA来研究其功能，我们发现慢病毒载体不会诱导海参中免疫相关酶的上调。然而,与基于dsRNA的RNA干扰（RNAi）方法相比，慢病毒介导的RNAi引起基因表达的动态变化。本研究为海参SRα的作用机理研究提供了技术支持。
