PDF(Pubmed)
Abstract：
OBJECTIVE: Our recent studies have indicated that trace copper co-existed with iron in hemosiderin particles of human genetic iron overload. To understand this phenomenon, we analyzed hemosiderin particles in iron-overloaded rat liver by using scanning transmission electron microscopy - energy-dispersive X-ray (STEM-EDX) spectroscopy.
METHODS: Samples for STEM-EDX spectroscopy were prepared from the liver of rats administered an intraperitoneal injection of dextran iron.
RESULTS: The micro-domain analysis with STEM-EDX spectroscopy showed that dense bodies contained high levels of iron and trace copper. Quantitative analysis of copper levels in the liver specimen using atomic spectrophotometry showed that copper concentration in the liver was not increased by iron overload. These findings suggest that the overload of iron induced distribution of trace copper to hemosiderin particles without changing cellular copper levels.
CONCLUSIONS: Co-existence of copper with iron was observed in hemosiderin particles of the liver of an experimental model of iron overload, suggesting that iron overload induced distribution of trace copper into hemosiderin particles.
METHODS: Samples for STEM-EDX spectroscopy were prepared from the liver of rats administered an intraperitoneal injection of dextran iron.
RESULTS: The micro-domain analysis with STEM-EDX spectroscopy showed that dense bodies contained high levels of iron and trace copper. Quantitative analysis of copper levels in the liver specimen using atomic spectrophotometry showed that copper concentration in the liver was not increased by iron overload. These findings suggest that the overload of iron induced distribution of trace copper to hemosiderin particles without changing cellular copper levels.
CONCLUSIONS: Co-existence of copper with iron was observed in hemosiderin particles of the liver of an experimental model of iron overload, suggesting that iron overload induced distribution of trace copper into hemosiderin particles.
摘要：
目的：我们最近的研究表明，人类遗传性铁过载的含铁血黄素颗粒中痕量铜与铁共存。为了理解这种现象，我们使用扫描透射电子显微镜-能量色散X射线（STEM-EDX）光谱法分析了铁过载大鼠肝脏中的含铁血黄素颗粒。
方法:从腹膜内注射右旋糖酐铁的大鼠的肝脏制备用于STEM-EDX光谱的样品。
结果：用STEM-EDX光谱进行的微域分析表明，致密体含有高水平的铁和痕量铜。使用原子分光光度法对肝脏标本中的铜水平进行定量分析表明，铁过载不会增加肝脏中的铜浓度。这些发现表明，铁的过载诱导了痕量铜在不改变细胞铜水平的情况下向含铁血黄素颗粒的分布。
结论：在铁过载实验模型的肝脏的含铁血黄素颗粒中观察到铜与铁的共存，表明铁过载导致痕量铜分布到含铁血黄素颗粒中。
方法:从腹膜内注射右旋糖酐铁的大鼠的肝脏制备用于STEM-EDX光谱的样品。
结果：用STEM-EDX光谱进行的微域分析表明，致密体含有高水平的铁和痕量铜。使用原子分光光度法对肝脏标本中的铜水平进行定量分析表明，铁过载不会增加肝脏中的铜浓度。这些发现表明，铁的过载诱导了痕量铜在不改变细胞铜水平的情况下向含铁血黄素颗粒的分布。
结论：在铁过载实验模型的肝脏的含铁血黄素颗粒中观察到铜与铁的共存，表明铁过载导致痕量铜分布到含铁血黄素颗粒中。
