PDF(Pubmed)
Abstract：
The extensive product and application of cadmium-quantum dots (Cd-QDs), one kind of semiconductor nanomaterials, lead to prolonged exposure to the environment. Cd-QDs have shown good properties in biomedical and imaging-related fields; the safety of Cd-QDs limits the application of these materials and technologies, however. The systematic distribution of CdTe QDs in organisms has been ascertained in previous studies. Nevertheless, it is relatively less reported about the toxicity of CdTe QDs to immune macromolecules and organs. Based on this, immunocytes (including lymphocyte subsets-CD4+ T and CD8+ T cells, splenocytes) and selenoprotein P (SelP) were chosen as targets for CdTe QDs immunotoxicity studies. Results indicate that CdTe QDs induced cytotoxicity to CD4+ T cells, CD8+ T cells and splenocytes by reducing cell viability and causing apoptosis as CdTe QDs and Cd2+ enter cells. At the molecular level, the direct interaction between CdTe QDs and SelP is proved by multispectral measurements, which demonstrated the alteration of protein structure. The combined results show that CdTe QDs induced adverse effects on the immune system at the cellular and molecular levels. This research contributes to a better understanding of CdTe QDs cause harmful damage to the immune system and provides new strategies for the inhibition and treatment of health damages caused by CdTe QDs.
摘要：
镉量子点(Cd-QDs)的广泛产品和应用,一种半导体纳米材料，导致长时间暴露在环境中。Cd-QDs在生物医学和成像相关领域表现出良好的性能,其安全性限制了这些材料和技术的应用,however.在先前的研究中已经确定了CdTe量子点在生物体中的系统分布。然而，关于CdTe量子点对免疫大分子和器官的毒性的报道相对较少。基于此,免疫细胞（包括淋巴细胞亚群-CD4+T和CD8+T细胞，选择脾细胞）和硒蛋白P（SelP）作为CdTeQDs免疫毒性研究的靶标。结果表明，CdTe量子点诱导对CD4+T细胞的细胞毒性，CD8+T细胞和脾细胞通过降低细胞活力和引起细胞凋亡作为CdTeQDs和Cd2+进入细胞。在分子水平上,多光谱测量证明了CdTe量子点与SelP之间的直接相互作用，这证明了蛋白质结构的改变。联合结果表明，CdTeQDs在细胞和分子水平上对免疫系统产生不利影响。这项研究有助于更好地了解CdTeQDs对免疫系统的有害损害，并为抑制和治疗CdTeQDs引起的健康损害提供了新的策略。
