PDF(Pubmed)
Abstract：
OBJECTIVE: Silver nanoparticles (AgNPs) are considered to be a very significant and intriguing type within the category of metallic nanoparticles, particularly in the context of their involvement in biological applications. The objective of this research is to use the green synthesis method in order to synthesize AgNPs by using the leaf extract of C. rotundata. Furthermore, the study aims to evaluate the antioxidant and anti-inflammatory properties of these nanoparticles.
METHODS: Fresh and healthy specimens of C. rotundata were gathered from Palk Bay, Tamil Nadu, India, and afterward subjected to a thorough washing process using tap water. The cleaned materials were air-dried and then fragmented into small bits and finely ground. The ethanolic extract of seagrass was then combined with a solution containing 1 millimolar (mM) silver nitrate (AgNo3). The decrease of silver ions in the solution was frequently measured using a UV-visible spectrophotometer. Synthesized AgNPs were investigated for antioxidants by DPPH (2,2-diphenyl-1-picrylhydrazyl) assay and anti-inflammatory activity was measured by protein-denaturation assay.
RESULTS: The use of C. rotundata leaf extract in the green synthesis of AgNPs, in the presence of 1 mM AgNO3, led to a noticeable alteration in the colour of the mixture, transitioning from a pale hue to a brown shade. This change in colour serves as evidence of the reduction of AgNo3 ions to silver ions, thereby facilitating the creation of AgNPs. The duration of the bio-reduction process of silver ions in the reaction mixture was observed to be two hours. The antioxidant and anti-inflammatory activity showed promising activity for AgNPs.
CONCLUSIONS: This study concluded that C. rotundata had antioxidant capabilities, and AgNPs derived from C. rotundata have potential use in pharmaceuticals and medication administration.
METHODS: Fresh and healthy specimens of C. rotundata were gathered from Palk Bay, Tamil Nadu, India, and afterward subjected to a thorough washing process using tap water. The cleaned materials were air-dried and then fragmented into small bits and finely ground. The ethanolic extract of seagrass was then combined with a solution containing 1 millimolar (mM) silver nitrate (AgNo3). The decrease of silver ions in the solution was frequently measured using a UV-visible spectrophotometer. Synthesized AgNPs were investigated for antioxidants by DPPH (2,2-diphenyl-1-picrylhydrazyl) assay and anti-inflammatory activity was measured by protein-denaturation assay.
RESULTS: The use of C. rotundata leaf extract in the green synthesis of AgNPs, in the presence of 1 mM AgNO3, led to a noticeable alteration in the colour of the mixture, transitioning from a pale hue to a brown shade. This change in colour serves as evidence of the reduction of AgNo3 ions to silver ions, thereby facilitating the creation of AgNPs. The duration of the bio-reduction process of silver ions in the reaction mixture was observed to be two hours. The antioxidant and anti-inflammatory activity showed promising activity for AgNPs.
CONCLUSIONS: This study concluded that C. rotundata had antioxidant capabilities, and AgNPs derived from C. rotundata have potential use in pharmaceuticals and medication administration.
摘要：
目的：银纳米粒子（AgNPs）被认为是金属纳米粒子类别中非常重要且有趣的类型，特别是在它们参与生物应用的背景下。本研究的目的是使用绿色合成方法，通过使用C.rotundata的叶提取物合成AgNPs。此外，该研究旨在评估这些纳米颗粒的抗氧化和抗炎特性。
方法：从PalkBay收集了新鲜和健康的C.rotundata标本，泰米尔纳德邦,印度,然后用自来水彻底清洗。将清洁的材料风干,然后破碎成小块并细磨。然后将海草的乙醇提取物与含有1毫摩尔(mM)硝酸银(AgNo3)的溶液混合。经常使用UV-可见分光光度计测量溶液中银离子的减少。通过DPPH（2,2-二苯基-1-吡啶酰肼）测定法研究了合成的AgNP的抗氧化剂，并通过蛋白质变性测定法测量了抗炎活性。
结果：在AgNPs的绿色合成中使用树枝叶提取物，在1mMAgNO3的存在下，导致混合物的颜色发生明显变化，从浅色过渡到棕色。这种颜色的变化是AgNo3离子还原成银离子的证据，从而促进AgNPs的创建。观察到反应混合物中银离子的生物还原过程的持续时间为2小时。抗氧化和抗炎活性对AgNPs显示出有希望的活性。
结论：这项研究得出结论，C.rotundata具有抗氧化能力，和来自C.rotundata的AgNPs在药物和药物管理中具有潜在的用途。
方法：从PalkBay收集了新鲜和健康的C.rotundata标本，泰米尔纳德邦,印度,然后用自来水彻底清洗。将清洁的材料风干,然后破碎成小块并细磨。然后将海草的乙醇提取物与含有1毫摩尔(mM)硝酸银(AgNo3)的溶液混合。经常使用UV-可见分光光度计测量溶液中银离子的减少。通过DPPH（2,2-二苯基-1-吡啶酰肼）测定法研究了合成的AgNP的抗氧化剂，并通过蛋白质变性测定法测量了抗炎活性。
结果：在AgNPs的绿色合成中使用树枝叶提取物，在1mMAgNO3的存在下，导致混合物的颜色发生明显变化，从浅色过渡到棕色。这种颜色的变化是AgNo3离子还原成银离子的证据，从而促进AgNPs的创建。观察到反应混合物中银离子的生物还原过程的持续时间为2小时。抗氧化和抗炎活性对AgNPs显示出有希望的活性。
结论：这项研究得出结论，C.rotundata具有抗氧化能力，和来自C.rotundata的AgNPs在药物和药物管理中具有潜在的用途。
