METHODS: Fresh and healthy specimens of C. rotundata were gathered from Palk Bay, Tamil Nadu, India, and afterward subjected to a thorough washing process using tap water. The cleaned materials were air-dried and then fragmented into small bits and finely ground. The ethanolic extract of seagrass was then combined with a solution containing 1 millimolar (mM) silver nitrate (AgNo3). The decrease of silver ions in the solution was frequently measured using a UV-visible spectrophotometer. Synthesized AgNPs were investigated for antioxidants by DPPH (2,2-diphenyl-1-picrylhydrazyl) assay and anti-inflammatory activity was measured by protein-denaturation assay.
RESULTS: The use of C. rotundata leaf extract in the green synthesis of AgNPs, in the presence of 1 mM AgNO3, led to a noticeable alteration in the colour of the mixture, transitioning from a pale hue to a brown shade. This change in colour serves as evidence of the reduction of AgNo3 ions to silver ions, thereby facilitating the creation of AgNPs. The duration of the bio-reduction process of silver ions in the reaction mixture was observed to be two hours. The antioxidant and anti-inflammatory activity showed promising activity for AgNPs.
CONCLUSIONS: This study concluded that C. rotundata had antioxidant capabilities, and AgNPs derived from C. rotundata have potential use in pharmaceuticals and medication administration.
方法:从PalkBay收集了新鲜和健康的C.rotundata标本,泰米尔纳德邦,印度,然后用自来水彻底清洗。将清洁的材料风干,然后破碎成小块并细磨。然后将海草的乙醇提取物与含有1毫摩尔(mM)硝酸银(AgNo3)的溶液混合。经常使用UV-可见分光光度计测量溶液中银离子的减少。通过DPPH(2,2-二苯基-1-吡啶酰肼)测定法研究了合成的AgNP的抗氧化剂,并通过蛋白质变性测定法测量了抗炎活性。
结果:在AgNPs的绿色合成中使用树枝叶提取物,在1mMAgNO3的存在下,导致混合物的颜色发生明显变化,从浅色过渡到棕色。这种颜色的变化是AgNo3离子还原成银离子的证据,从而促进AgNPs的创建。观察到反应混合物中银离子的生物还原过程的持续时间为2小时。抗氧化和抗炎活性对AgNPs显示出有希望的活性。
结论:这项研究得出结论,C.rotundata具有抗氧化能力,和来自C.rotundata的AgNPs在药物和药物管理中具有潜在的用途。