Abstract：
Trypanosomatids are obligatory parasites, some of which are responsible for important human and animal diseases, but the vast majority of trypanosomatids are restricted to invertebrate hosts. Isolation and in vitro cultivation of trypanosomatids from insect hosts enable their description, characterization, and subsequently genetic and genomic studies. However, exact nutritional requirements are still unknown for most trypanosomatids and thus very few defined media are available. This mini review provides information about the role of different ingredients, recommendations and advice on essential supplements and important physicochemical parameters of culture media with the aim of facilitating first attempts to cultivate insect-infesting trypanosomatids, with a focus on monoxenous trypanosomatids.
摘要：
锥虫是强制性寄生虫，其中一些是重要的人类和动物疾病的原因，但是绝大多数锥虫只限于无脊椎动物宿主。从昆虫宿主中分离和体外培养锥虫，可以对其进行描述，表征，以及随后的遗传和基因组研究。然而,大多数锥虫的确切营养需求仍然未知，因此很少有确定的培养基可用。这个迷你评论提供了有关不同成分的作用的信息，关于培养基的基本补充剂和重要物理化学参数的建议和建议，目的是促进首次尝试培育昆虫侵染锥虫，专注于单生锥虫。
