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Abstract：
To learn about the gene structure, phylogenetic evolution, and function under biotic and abiotic stresses of BTB (Bric-a-Brac/Tramtrack/Broad Complex) genes in Paulownia fortunei, a whole-genome sequence evaluation was carried out, and a total of 62 PfBTB genes were identified. The phylogenetic analysis showed that PfBTB proteins are divided into eight groups, and these proteins are highly conserved. PfBTB genes were unevenly distributed on 17 chromosomes. The colinearity analysis found that fragment replication and tandem replication are the main modes of gene amplification in the PfBTB family. The analysis of cis-acting elements suggests that PfBTB genes may be involved in a variety of biological processes. The transcriptomic analysis results showed that PfBTB3/12/14/16/19/36/44 responded to Paulownia witches\' broom (PaWB), while PfBTB1/4/17/43 responded to drought stress, and the RT-qPCR results further support the reliability of transcriptome data. In addition, the association analysis between miRNA and transcriptome revealed a 91-pair targeting relationship between miRNAs and PfBTBs. In conclusion, the BTB genes in Paulownia are systematically identified in this research. This work provides useful knowledge to more fully appreciate the potential functions of these genes and their possible roles in the occurrence of PaWB and in response to stress.
摘要：
要了解基因结构，系统发育进化，泡桐中BTB（Bric-a-Brac/Tramtrack/BroadComplex）基因在生物和非生物胁迫下的功能，进行了全基因组序列评估，共鉴定出62个PfBTB基因。系统发育分析表明，PfBTB蛋白分为八组，这些蛋白质是高度保守的。PfBTB基因在17条染色体上分布不均。共线性分析发现，片段复制和串联复制是PfBTB家族基因扩增的主要模式。顺式作用元件的分析表明PfBTB基因可能参与多种生物学过程。转录组学分析结果显示，PfBTB3/12/14/16/19/36/44对泡桐女巫扫帚（PaWB）有反应，而PfBTB1/4/17/43响应干旱胁迫，RT-qPCR结果进一步支持转录组数据的可靠性。此外,miRNA和转录组之间的关联分析揭示了miRNA和PfBTB之间的91对靶向关系.总之,本研究系统地鉴定了泡桐中的BTB基因。这项工作提供了有用的知识，可以更充分地了解这些基因的潜在功能及其在PaWB的发生和对压力的反应中的可能作用。
