PDF(Pubmed)
Abstract：
Pichinde virus (PICV) can infect several animal species and has been developed as a safe and effective vaccine vector. Our previous study showed that pigs vaccinated with a recombinant PICV-vectored vaccine expressing the hemagglutinin (HA) gene of an H3N2 influenza A virus of swine (IAV-S) developed virus-neutralizing antibodies and were protected against infection by the homologous H3N2 strain. The objective of the current study was to evaluate the immunogenicity and protective efficacy of a trivalent PICV-vectored vaccine expressing HA antigens from the three co-circulating IAV-S subtypes: H1N1, H1N2, and H3N2. Pigs immunized with the trivalent PICV vaccine developed virus-neutralizing (VN) and hemagglutination inhibition (HI) antibodies against all three matching IAV-S. Following challenge infection with the H1N1 strain, five of the six pigs vaccinated with the trivalent vaccine had no evidence of IAV-S RNA genomes in nasal swabs and bronchoalveolar lavage fluid, while all non-vaccinated control pigs showed high number of copies of IAV-S genomic RNA in these two types of samples. Overall, our results demonstrate that the trivalent PICV-vectored vaccine elicits antibody responses against the three targeted IAV-S strains and provides protection against homologous virus challenges in pigs. Therefore, PICV exhibits the potential to be explored as a viral vector for delivering multiple vaccine antigens in swine.
摘要：
Pichinde病毒（PICV）可以感染多种动物，已被开发为安全有效的疫苗载体。我们先前的研究表明，用表达猪H3N2甲型流感病毒（IAV-S）的血凝素（HA）基因的重组PICV载体疫苗接种的猪产生了病毒中和抗体，并受到同源H3N2株的感染保护。本研究的目的是评估表达来自三种共同循环的IAV-S亚型:H1N1、H1N2和H3N2的HA抗原的三价PICV载体疫苗的免疫原性和保护效力。用三价PICV疫苗免疫的猪开发了针对所有三种匹配的IAV-S的病毒中和（VN）和血凝抑制（HI）抗体。在用H1N1毒株攻击感染后，接种三价疫苗的六只猪中的五只在鼻拭子和支气管肺泡灌洗液中没有IAV-SRNA基因组的证据，而所有未接种疫苗的对照猪在这两种类型的样品中显示高拷贝数的IAV-S基因组RNA。总的来说,我们的结果表明,三价PICV载体疫苗可引发针对3种靶向IAV-S毒株的抗体应答,并在猪中提供针对同源病毒攻击的保护.因此,PICV显示出作为在猪中递送多种疫苗抗原的病毒载体被探索的潜力。
