PDF(Pubmed)
Abstract：
The research presents data from a preclinical study on the anti-inflammatory effects of a sodium dichloroacetate and sodium valproate combination (DCA-VPA). The 2-week treatment with a DCA 100 mg/kg/day and VPA 150 mg/kg/day combination solution in drinking water\'s effects on the thymus weight, its cortex/medulla ratio, Hassall\'s corpuscles (HCs) number in the thymus medulla, and the expression of inflammatory and immune-response-related genes in thymocytes of male Balb/c mice were studied. Two groups of mice aged 6-7 weeks were investigated: a control (n = 12) and a DCA-VPA-treated group (n = 12). The treatment did not affect the body weight gain (p > 0.05), the thymus weight (p > 0.05), the cortical/medulla ratio (p > 0.05), or the number of HCs (p > 0.05). Treatment significantly increased the Slc5a8 gene expression by 2.1-fold (p < 0.05). Gene sequence analysis revealed a significant effect on the expression of inflammation-related genes in thymocytes by significantly altering the expression of several genes related to the cytokine activity pathway, the inflammatory response pathway, and the Il17 signaling pathway in thymocytes. Data suggest that DCA-VPA exerts an anti-inflammatory effect by inhibiting the inflammatory mechanisms in the mouse thymocytes.
摘要：
该研究提供了来自二氯乙酸钠和丙戊酸钠组合（DCA-VPA）的抗炎作用的临床前研究的数据。在饮用水中使用DCA100mg/kg/天和VPA150mg/kg/天组合溶液的2周治疗对胸腺重量的影响，它的皮质/髓质比例，胸腺髓质中的Hassall小体（HCs）数，研究了雄性Balb/c小鼠胸腺细胞炎症和免疫反应相关基因的表达。研究了两组6-7周龄的小鼠:对照组(n=12)和DCA-VPA治疗组(n=12)。治疗不影响体重增加（p>0.05），胸腺重量（p>0.05），皮质/髓质比（p>0.05），或HC的数量(p>0.05)。处理使Slc5a8基因表达显著增加2.1倍(p<0.05)。基因序列分析显示，通过显着改变与细胞因子活性途径相关的几个基因的表达，对胸腺细胞中炎症相关基因的表达有显着影响，炎症反应途径，和胸腺细胞中的Il17信号通路。数据表明，DCA-VPA通过抑制小鼠胸腺细胞的炎症机制而发挥抗炎作用。
