PDF(Pubmed)
Abstract：
Nanofiber (NF) products exhibit outstanding performances in materials science, textiles, and medicine that cannot be realized using conventional technologies. However, the safety of such products is debated because of the potential health risks that nanomaterials pose and the lack of standardized guidelines for the safety evaluation of NF products. The global safety evaluations of nanomaterials have focused on evaluating the cytotoxicity of low-dimensional materials, including nanoparticles and nanotubes, based on OECD (Organization for Economic Co-operation and Development) criteria. NFs are one-dimensional materials with nanometer diameters and considerable lengths. Many fibers are applied in a densely woven web-like form, so assessing cellular penetration and fiber toxicity using the same methods is inappropriate. This study verifies the safety of the polyurethane (PU) and polyvinylidene fluoride (PVDF) polymers currently applied in filters and masks. To this end, polymer NFs were collected from each product, and the NFs were compared with reference samples using FT-IR and Raman spectroscopy. For the safety evaluation, DMSO stocks of varying concentrations of PVDF and PU NFs (at 0.5, 1, 5, and 10 μg/mL) were prepared. The cytotoxicity and inhibitory effects on nitric oxide production and protein expression obtained via Western blot were identified.
摘要：
纳米纤维（NF）产品在材料科学中表现突出，纺织品,以及使用常规技术无法实现的医学。然而,由于纳米材料带来的潜在健康风险以及缺乏NF产品安全性评估的标准化指南，此类产品的安全性存在争议。纳米材料的全球安全性评价主要集中在低维材料的细胞毒性评价上,包括纳米粒子和纳米管，根据OECD(经济合作与发展组织)标准。NF是具有纳米直径和相当大长度的一维材料。许多纤维以密集编织的网状形式应用，因此，使用相同的方法评估细胞渗透和纤维毒性是不合适的。这项研究验证了目前在过滤器和口罩中使用的聚氨酯（PU）和聚偏二氟乙烯（PVDF）聚合物的安全性。为此,从每种产品中收集聚合物NF，并使用FT-IR和拉曼光谱将NFs与参考样品进行比较。对于安全性评估，制备不同浓度的PVDF和PUNF(0.5、1、5和10μg/mL)的DMSO储备液。鉴定了通过Westernblot获得的细胞毒性和对一氧化氮产生和蛋白质表达的抑制作用。
