PDF(Pubmed)
Abstract：
Tuberculosis (TB) is a worldwide burden whose total control and eradication remains a challenge due to factors including false positive/negative diagnoses associated with the poor sensitivity of the current diagnostics in immune-compromised and post-vaccinated individuals. As these factors complicate both diagnosis and treatment, the early diagnosis of TB is of pivotal importance towards reaching the universal vision of a TB-free world. Here, an aptasensor for signaling an interferon gamma (IFN-γ) TB biomarker at low levels is reported. The aptasensor was assembled through gold-thiol interactions between poly(3,4-propylenedioxythiophene), gold nanoparticles, and a thiol-modified DNA aptamer specific to IFN-γ. The aptasensor sensitively detected IFN-γ in spiked pleural fluid samples with a detection limit of 0.09 pg/mL within a linear range from 0.2 pg/mL to 1.2 pg/mL. The good performance of the reported aptasensor indicates that it holds the potential for application in the early diagnosis of, in addition to TB, various diseases associated with IFN-γ release in clinical samples.
摘要：
结核病(TB)是世界范围内的负担,其完全控制和根除由于包括与免疫受损和接种后个体中的当前诊断的差的灵敏度相关的假阳性/阴性诊断的因素而仍然是挑战。由于这些因素使诊断和治疗复杂化，结核病的早期诊断对于实现无结核病世界的普遍愿景至关重要。这里,据报道，低水平的干扰素γ（IFN-γ）TB生物标志物的信号传递是aptasensor。aptasensor是通过聚（3,4-亚丙基二氧噻吩）之间的金-硫醇相互作用组装的，金纳米粒子，和对IFN-γ具有特异性的巯基修饰的DNA适体。在0.2pg/mL至1.2pg/mL的线性范围内，aptasensor灵敏地检测了加标胸膜液样品中的IFN-γ，检出限为0.09pg/mL。报告的aptasensor的良好性能表明，它具有应用于早期诊断的潜力，除了结核病，临床样品中与IFN-γ释放相关的各种疾病。
