PDF(Pubmed)
Abstract：
Antibiotic-free approaches are more important than ever to address the rapidly growing problem of the antibiotic resistance crisis. The photolysis of the bacterial virulence factor staphyloxanthin using blue light at 460 nm (BL460 nm) has been found to effectively attenuate Staphylococcus aureus to chemical and physical agents. However, phototherapy using BL640 nm still needs to be investigated in detail for its safety in eradicating Staphylococcus aureus in vitro and in vivo. In this study, we employed a 460 nm continuous-wavelength LED source and a low concentration of hydrogen peroxide to treat S. aureus under a culturing condition and a wound abrasion mouse model. The results demonstrated the safety of the combined therapy when it did not modify the bacterial virulence factors or the susceptibility to widely used antibiotics. In addition, the results of the mouse model also showed that the combined therapy was safe to apply to mouse skin since it did not cause adverse skin irritation. More importantly, the therapy can aid in healing S. aureus-infected wounds with an efficacy comparable to that of the topical antibiotic Fucidin. The aforementioned findings indicate that the concurrent application of BL460 nm and hydrogen peroxide can be used safely as an alternative or adjunct to antibiotics in treating S. aureus-infected wounds.
摘要：
对于解决快速增长的抗生素耐药性危机问题，无抗生素方法比以往任何时候都更为重要。已发现使用460nm(BL460nm)的蓝光对细菌毒力因子黄黄质的光解有效地将金黄色葡萄球菌减弱为化学和物理试剂。然而,使用BL640nm的光疗仍需要详细研究其在体外和体内根除金黄色葡萄球菌的安全性。在这项研究中,我们使用460nm连续波长LED光源和低浓度的过氧化氢在培养条件和伤口擦伤小鼠模型下治疗金黄色葡萄球菌.结果表明，当联合疗法不改变细菌毒力因子或对广泛使用的抗生素的敏感性时，联合疗法的安全性。此外,小鼠模型的结果还表明，该联合疗法适用于小鼠皮肤是安全的，因为它不会引起不良的皮肤刺激。更重要的是,该疗法可以帮助治愈金黄色葡萄球菌感染的伤口，其功效与局部抗生素Fucidin相当。上述发现表明,在治疗金黄色葡萄球菌感染的伤口中,同时应用BL460nm和过氧化氢可以安全地用作抗生素的替代或辅助。
