PDF(Pubmed)
Abstract：
Human B cell immortalization that maintains the constant growth characteristics and antibody expression of B cells in vitro is very critical for the development of antibody drugs and products for the diagnosis and bio-therapeutics of human diseases. Human B cell immortalization methods include Epstein-Barr virus (EBV) transformation, Simian virus 40 (SV40) virus infection, in vitro genetic modification, and activating CD40, etc. Immortalized human B cells produce monoclonal antibodies (mAbs) very efficiently, and the antibodies produced in this way can overcome the immune rejection caused by heterologous antibodies. It is an effective way to prepare mAbs and an important method for developing therapeutic monoclonal antibodies. Currently, the US FDA has approved more than 100 mAbs against a wide range of illnesses such as cancer, autoimmune diseases, infectious diseases, and neurological disorders. This paper reviews the research progress of human B cell immortalization, its methods, and future directions as it is a powerful tool for the development of monoclonal antibody preparation technology.
摘要：
在体外维持B细胞的恒定生长特征和抗体表达的人B细胞永生化对于开发用于人类疾病诊断和生物治疗的抗体药物和产品非常关键。人B细胞永生化方法包括EB病毒(EBV)转化,猿猴病毒40（SV40）病毒感染，体外遗传修饰，和激活CD40等。永生化人B细胞非常有效地产生单克隆抗体（mAb），以这种方式产生的抗体可以克服异源抗体引起的免疫排斥。它是制备单克隆抗体的有效途径和开发治疗性单克隆抗体的重要方法。目前,美国FDA已经批准了100多种单克隆抗体，用于治疗癌症等多种疾病。自身免疫性疾病，传染病,和神经系统疾病。本文就人类B细胞永生化的研究进展作一综述,其方法,以及未来的发展方向，因为它是发展单克隆抗体制备技术的有力工具。
