PDF(Pubmed)
Abstract：
Subchondral bone cysts in horses represent one of the main causes of lameness that can occur in different anatomical locations. The study describes the treatment in regenerative therapy of the intracystic implantation of adipose tissue mesenchymal stromal cells (AMSCs) included in platelet-rich plasma (PRP). The ability of AMSCs to differentiate in osteogenic cells was tested in vitro and in vivo. Given the aim to investigate the application of AMSCs in bone defects and orthopedic pathologies in horses, a four-year-old male thoroughbred racing horse that had never raced before was treated for lameness of the left hind leg caused by a cyst of the medial femoral condyle. The horse underwent a new surgery performed with an arthroscopic approach in which the cystic cavity was filled with AMSCs contained in the PRP. Radiographs were taken 3, 5, and 10 months after the surgery to assess the development of newly regenerated bone tissue in the gap left by the cyst. Twelve months after the operation and after six months of regular daily training, the horse did not show any symptoms of lameness and started a racing career. According to the study, the use of AMSCs and PRP suggests promising benefits for treating subchondral bone cysts.
摘要：
马的软骨下骨囊肿是可能发生在不同解剖位置的跛行的主要原因之一。该研究描述了包含在富含血小板的血浆（PRP）中的脂肪组织间充质基质细胞（AMSC）的囊内植入的再生疗法。在体外和体内测试了AMSCs在成骨细胞中的分化能力。鉴于旨在研究AMSCs在马骨缺损和骨科病理中的应用，一匹从未参加过比赛的四岁雄性纯种赛马因股骨内侧髁囊肿导致左后腿跛行而接受治疗。这匹马接受了用关节镜入路进行的新手术，其中囊腔充满了PRP中所含的AMSC。手术后3、5和10个月进行放射学检查，以评估囊肿留下的间隙中新再生的骨组织的发育。术后12个月和经过6个月的常规日常训练，这匹马没有表现出任何跛行症状，开始了赛车生涯。根据研究,AMSCs和PRP的使用提示对于治疗软骨下骨囊肿有希望的益处.
