PDF(Pubmed)
Abstract：
(1) Background: The human MutS homolog, hMSH2, is known to be involved in DNA mismatch repair and is responsible for maintaining the stability of the genome. When DNA damage occurs, MSH2 promotes cell apoptosis via the regulation of ATR/Chk2/p53 signal transduction, and MSH2 deficiency is also related to accelerated telomere shortening in humans. MSH2 missense mutations are involved in a defective DNA reparation process, and it can be implied in carcinogenesis, as it is already involved in well-known cancer-related syndromes such as Lynch syndrome. Human MSH6, which stands for mutS homolog 6, is a member of the MMR family that is responsible for the repair of post-replicative mismatched DNA bases. It is also one of the proteins with gene mutations that are associated with a high risk of developing Lynch syndrome, leading to a large series of tumors. (2) Methods: Patients and their clinical and pathological features were selected from the database of the project GRAPHSENSGASTROINTES and used accordingly, with ethics committee approval no. 32647/2018 awarded by the County Emergency Hospital from Targu-Mures. Analyses were conducted on whole blood, saliva, urine, and tumoral tissue samples using a stochastic method with stochastic microsensors. (3) Results: The results obtained using stochastic sensors were correlated with a series of macroscopic and microscopic pathological features for each sample type. Criteria or relationships were established for tumor location, vascular and perineural invasions, lymph node metastases, the presence of tumor deposits, and the presence of a mucus compound in the tumor mass. (4) Conclusions: The correlation between the concentrations of MSH2 in the four types of samples and the pathological features allowed for the fast characterization of a tumor, which can help surgeons and oncologists choose personalized treatments. Also, the colorectal tumor location was correlated with the concentration of MSH2 in whole blood, urine, and saliva. MSH6, which stands for mutS homolog 6, is not only useful in immunohistochemistry but in pathology practice as well. In this paper, the relationships between MSH6 levels in four biological fluids-whole blood, saliva, urine, and tissues-and tumor locations among the colorectal area, gross features, presence of a mucinous compound, molecular subtype, stroma features, and vascular invasions are presented.
摘要：
（1）背景：人类MutS同源物，已知hMSH2参与DNA错配修复并且负责维持基因组的稳定性。当DNA损伤发生时,MSH2通过调节ATR/Chk2/p53信号转导促进细胞凋亡,MSH2缺乏也与人类端粒缩短加速有关。MSH2错义突变参与有缺陷的DNA修复过程，它可以暗示致癌作用，因为它已经参与了众所周知的癌症相关综合征，如Lynch综合征。人MSH6代表mutS同源物6，是MMR家族的成员，负责修复复制后错配的DNA碱基。它也是具有基因突变的蛋白质之一，与患Lynch综合征的高风险相关，导致一系列的肿瘤.(2)方法:从GRAPHSENSGASTROINTES项目的数据库中选择患者及其临床和病理特征,经伦理委员会批准。32647/2018由县急诊医院从Targu-Mures授予。对全血进行了分析，唾液,尿液,和肿瘤组织样本使用随机微传感器的随机方法。（3）结果：使用随机传感器获得的结果与每种样品类型的一系列宏观和微观病理特征相关。建立了肿瘤定位的标准或关系，血管和神经周浸润，淋巴结转移,肿瘤沉积物的存在，和肿瘤块中存在粘液化合物。（4）结论：四种类型的样品中MSH2的浓度与病理特征之间的相关性允许肿瘤的快速表征，这可以帮助外科医生和肿瘤学家选择个性化的治疗方法。此外，结直肠肿瘤部位与全血中MSH2浓度相关,尿液,还有唾液.MSH6代表mutS同源物6，不仅在免疫组织化学中有用，而且在病理学实践中也有用。在本文中,四种生物体液-全血MSH6水平之间的关系，唾液,尿液,结直肠区域的组织和肿瘤位置，总体特征，粘液化合物的存在，分子亚型,基质特征,和血管浸润。
