PDF(Pubmed)
Abstract：
Proanthocyanidins (Pros), a natural polyphenolic compound found in grape seed and other plants, have received significant attention as additives in animal feed. However, the specific mechanism by which Pros affect fish health remains unclear. Therefore, the aim of this study was to investigate the potential effects of dietary Pro on common carp by evaluating biochemical parameters and multi-omics analysis. The results showed that Pro supplementation improved antioxidant capacity and the contents of polyunsaturated fatty acids (n-3 and n-6) and several bioactive compounds. Transcriptomic analysis demonstrated that dietary Pro caused an upregulation of the sphingolipid catabolic process and the lysosome pathway, while simultaneously downregulating intestinal cholesterol absorption and the PPAR signaling pathway in the intestines. Compared to the normal control (NC) group, the Pro group exhibited higher diversity in intestinal microbiota and an increased relative abundance of Cetobacterium and Pirellula. Furthermore, the Pro group had a lower Firmicutes/Bacteroidetes ratio and a decreased relative abundance of potentially pathogenic bacteria. Collectively, dietary Pro improved antioxidant ability, muscle nutrients, and the diversity and composition of intestinal microbiota. The regulation of lipid metabolism and improvement in muscle nutrients were linked with changes in the intestinal microbiota.
摘要：
原花青素（Pro），一种在葡萄籽和其他植物中发现的天然多酚化合物，作为动物饲料中的添加剂受到了极大的关注。然而,Pros影响鱼类健康的具体机制尚不清楚。因此,这项研究的目的是通过评估生化参数和多组学分析来研究饲粮Pro对鲤鱼的潜在影响。结果表明，补充Pro可以提高抗氧化能力，提高多不饱和脂肪酸（n-3和n-6）和几种生物活性化合物的含量。转录组分析表明，膳食Pro引起鞘脂分解代谢过程和溶酶体途径的上调，同时下调肠道胆固醇吸收和肠道PPAR信号通路。与正常对照组(NC)相比,Pro组表现出更高的肠道微生物群多样性和相对丰度增加的Cetobacterium和Pirellula。此外，Pro组的Firmicutes/拟杆菌比例较低,潜在致病菌的相对丰度降低.总的来说，膳食Pro提高抗氧化能力，肌肉营养素，以及肠道菌群的多样性和组成。脂质代谢的调节和肌肉营养素的改善与肠道微生物群的变化有关。
