Abstract：
Marek\'s disease is a contagious proliferative disease of chickens caused by an alphaherpesvirus called Marek\'s disease virus. A bivalent mRNA vaccine encoding MDV\'s glycoprotein-B and phosphoprotein-38 antigens was synthesized and encapsulated in lipid nanoparticles. Tumor incidence, lesion score, organ weight indices, MDV genome load and cytokine expression were used to evaluate protection and immunostimulatory effects of the tested mRNA vaccine after two challenge trials. Results from the first trial showed decreased tumor incidence and a reduction in average lesion scores in chickens that received the booster dose. The second trial demonstrated that vaccination with the higher dose of the vaccine (10 μg) significantly decreased tumor incidence, average lesion scores, bursal atrophy, and MDV load in feather tips when compared to the controls. Changes in expression of type I and II interferons suggested a possible role for these cytokines in initiation and maintenance of the vaccine-originated immune responses.
摘要：
马立克氏病是由称为马立克氏病病毒的α疱疹病毒引起的鸡的传染性增殖性疾病。合成了编码MDV糖蛋白-B和磷蛋白-38抗原的二价mRNA疫苗，并将其包封在脂质纳米颗粒中。肿瘤发病率，病变评分,器官重量指数,在两次攻击试验后，使用MDV基因组负荷和细胞因子表达来评估测试的mRNA疫苗的保护和免疫刺激作用。第一次试验的结果表明，接受加强剂量的鸡的肿瘤发病率降低，平均病变评分降低。第二项试验表明，用较高剂量的疫苗（10μg）接种疫苗可显着降低肿瘤发病率，平均病变评分,法氏囊萎缩,与对照相比，羽毛提示中的MDV负荷。I型和II型干扰素表达的变化表明这些细胞因子在疫苗起源的免疫应答的启动和维持中可能的作用。
