Abstract：
The reproductive cycle of equines tends to be seasonal and is influenced by factors such as light and temperature. The process and methods of regulating the mare oestrous cycle in the anestrus period are still immature. The effects of noncoding RNAs and mRNAs on the oestrous cycle have aroused much interest, but corresponding analyses of seasonal mare ovaries have not been reported. Here, we report a whole transcriptome analysis of the Mongolian horse ovarian cortex collected in anestrus and diestrus periods. In total, 1081 mRNAs, 205 lncRNAs, 54 circRNAs, and 13 miRNAs were upregulated in winter anestrus ovarian cortex (WAO), and 1261 mRNAs, 90 lncRNAs, 29 circRNAs, and 40 miRNAs were upregulated in summer diestrus ovarian cortex (SDO). The GO and KEGG enrichment analysis of differentially expressed mRNAs and target genes of differentially expressed lncRNAs, circRNAs, and miRNAs revealed some key functions and pathways that may be related to follicle and oocyte development. We found that estrogen-related pathways were enriched in different RNAs. Our data were used to generate miRNA, circRNA, lncRNA, and mRNA databases from the Mongolian horse ovary and differential expression profiles between WAO and SDO; these results provide clues for exploring methods of estrus regulation in mares during the anestrus period.
摘要：
马的生殖周期往往是季节性的，并且受到光照和温度等因素的影响。在发情期调节母马发情周期的过程和方法仍不成熟。非编码RNA和mRNA对发情周期的影响引起了人们的极大兴趣,但对季节性母马卵巢的相应分析尚未报道。这里,我们报告了在发情期和发情期收集的蒙古马卵巢皮质的整体转录组分析。总的来说,1081个mRNA,205个lncRNAs,54个circRNAs，13个miRNAs在冬季反流卵巢皮质（WAO）中上调，和1261个mRNA，90个lncRNAs,29个circRNAs，夏季动情卵巢皮质（SDO）中40个miRNAs上调。差异表达mRNA和差异表达lncRNAs靶基因的GO和KEGG富集分析,circRNAs,miRNAs揭示了一些可能与卵泡和卵母细胞发育有关的关键功能和途径。我们发现雌激素相关途径富含不同的RNA。我们的数据被用来生成miRNA，circRNA,lncRNA,和来自蒙古马卵巢的mRNA数据库以及WAO和SDO之间的差异表达谱；这些结果为探索发情期母马的发情调节方法提供线索。
