Abstract：
Mechanical loading can play a critical role in bone modeling/remodeling through osteoblasts, with several factors being involved in this process.The present study aims to systematically review the effect of mechanical stimulation on human osteoblast cell lineage combined with other variables.The PubMed and Scopus databases were electronically searched for studies analyzing the effect of compression and tension on human osteoblasts at different differentiation stages. Studies that used carcinogenic osteoblasts were excluded. In addition, studies that did not analyze the osteogenic differentiation or proliferation of cells were excluded. The risk of bias of the studies was evaluated using the modified CONSORT (Consolidated Standards of Reporting Trials) checklist. a total of 20 studies were included. The cells were subjected to tension and compression in 5 and 15 studies, respectively. The application of uniaxial and cyclic strain increased the proliferation of osteoblasts. The same increased pattern could be observed for the osteogenesis of the cells. The impact of the tensile force on the expression of the osteoclastic markers differed based on the loading characteristics. On the other side, the impact of compression on the proliferation of osteoblasts varied according to the magnitude and duration of the force. Besides, different patterns of alternations were observed among the osteogenic markers in response to compression. Meanwhile, compression increased the expression of the osteoclastic markers. It has been shown that the response of the markers related to bone formation or resorption can be altered based on the differentiation stage of the cells, the cell culture system, and the magnitude and duration of the force.
摘要：
机械负荷可以通过成骨细胞在骨建模/重塑中起关键作用，这个过程涉及几个因素。本研究旨在系统回顾机械刺激对人类成骨细胞谱系的影响，并结合其他变量。以电子方式搜索PubMed和Scopus数据库，以进行分析压缩和张力对不同分化阶段的人成骨细胞的影响的研究。排除了使用致癌成骨细胞的研究。此外,未分析细胞成骨分化或增殖的研究被排除.使用修改后的CONSORT（合并报告试验标准）清单评估研究的偏倚风险。共纳入20项研究.在5和15项研究中，细胞受到拉伸和压缩，分别。单轴和循环应变的应用增加了成骨细胞的增殖。对于细胞的成骨作用，可以观察到相同的增加模式。拉力对破骨细胞标志物表达的影响根据载荷特性而有所不同。在另一边,压缩对成骨细胞增殖的影响根据力的大小和持续时间而变化。此外,在响应压缩的成骨标志物之间观察到不同的交替模式。同时,压缩增加了破骨细胞标志物的表达。已经表明，与骨形成或吸收相关的标志物的反应可以根据细胞的分化阶段而改变，细胞培养系统,以及力的大小和持续时间。
