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Abstract：
Procalcitonin (PCT) and protein carbonylated content (PCC) are promising biomarkers for bacterial infection and inflammation in veterinary medicine. This study examined plasma PCT and PCC levels in healthy cows (H) and cows with subclinical mastitis (SCM). A total of 130 cows (65 H and 65 SCM) were included in this study. Blood samples were collected, and plasma was frozen at -80 °C. PCT levels were determined using a bovine procalcitonin ELISA kit, while PCC was measured following the methodology of Levine et al. Statistical analysis revealed a significant difference in PCT levels between H (75.4 pg/mL) and SCM (107.3 pg/mL) cows (p < 0.001) and significantly lower concentrations of PCC in the SCM group (H: 0.102 nmol/mL/mg, SCM: 0.046 nmol/mL/mg; p < 0.001). The PCT cut-off value for distinguishing healthy and subclinical mastitis animals was >89.8 pg/mL (AUC 0.695), with a sensitivity of 66.2% and specificity of 69.2%. PCT showed potential value as a diagnostic tool to help in decision making for subclinical mastitis cases, while PCC requires further studies to investigate the trend of this biomarker during localized pathology.
摘要：
降钙素原（PCT）和蛋白质羰基化含量（PCC）是兽医学中细菌感染和炎症的有前途的生物标志物。这项研究检查了健康奶牛（H）和患有亚临床乳腺炎（SCM）的奶牛的血浆PCT和PCC水平。本研究包括总共130头母牛(65H和65SCM)。采集血样,血浆在-80℃冷冻。使用牛降钙素原ELISA试剂盒测定PCT水平，而PCC是按照Levine等人的方法测量的。统计分析显示，H（75.4pg/mL）和SCM（107.3pg/mL）母牛之间的PCT水平存在显着差异（p<0.001），并且SCM组中PCC的浓度显着降低（H：0.102nmol/mL/mg，SCM:0.046nmol/mL/mg;p<0.001)。区分健康和亚临床乳腺炎动物的PCT临界值>89.8pg/mL（AUC0.695），敏感性为66.2%，特异性为69.2%。PCT显示出作为诊断工具的潜在价值，可以帮助亚临床乳腺炎病例的决策。而PCC需要进一步的研究来调查这种生物标志物在局部病理过程中的趋势。
