PDF(Pubmed)
Abstract：
This study investigates the intricate dynamics of matrix stiffness, substrate composition, and cell-cell interactions and elucidates their cumulative effects on fibroblast behavior in different culture contexts. Three primary substrate types were examined: non-coated, collagen-coated, and collagen hydrogel, within both two-dimensional (2D) monolayer and three-dimensional (3D) spheroid cultures. The research provides several key insights. First, 3D spheroid culture, which promotes robust cell-cell interactions, emerges as a critical factor in maintaining fibroblast functionality. Second, substrate stiffness significantly influences results, with the soft collagen hydrogel showing superior support for fibroblast function. Notably, fibroblasts cultured on collagen hydrogel in 2D exhibit comparable functionality to those in 3D, highlighting the importance of substrate mechanical properties. Third, surface composition, as exemplified by collagen coating, showed a limited effect compared to the other factors studied. These findings provide a basis for innovative applications in regenerative medicine, tissue engineering, and drug testing models, and offer valuable insights into harnessing the potential of fibroblasts and advancing biomedical sciences.
摘要：
这项研究调查了矩阵刚度的复杂动力学，基材组成,和细胞-细胞相互作用，并阐明了它们在不同培养环境中对成纤维细胞行为的累积影响。检查了三种主要的基材类型：非涂层，胶原蛋白涂层，和胶原蛋白水凝胶，在二维（2D）单层和三维（3D）球体培养中。该研究提供了几个关键见解。首先,3D球体培养，促进强大的细胞间相互作用，成为维持成纤维细胞功能的关键因素。第二,基板刚度显著影响结果，软胶原水凝胶显示出成纤维细胞功能的优越支持。值得注意的是,在2D中在胶原水凝胶上培养的成纤维细胞表现出与3D中相当的功能，强调基材机械性能的重要性。第三，表面成分,例如胶原蛋白涂层，与研究的其他因素相比，效果有限。这些发现为再生医学的创新应用提供了基础。组织工程,和药物测试模型，并为利用成纤维细胞的潜力和推进生物医学科学提供有价值的见解。
