Abstract：
Polygonum multiflorum Thunb. is a traditional Chinese medicine with extensive distribution and robust adaptability, but comprehensive research on its acid and alkali resistance is presently lacking. This study aimed to analyze the effects of 5 months of continuous pH stress on the physiological and photosynthetic parameters of P. multiflorum, and the content of effective components. Results revealed that pH stress significantly influenced the normal growth, physiological functions, and photosynthetic indicators of P. multiflorum. At soil pH 4.5, the tubers of P. multiflorum exhibited the highest levels of 2,3,5,4\'-tetrahydroxy stilbene-2-O-β-d-glucoside (THSG) and total anthraquinones at 5.41% and 0.38%, respectively. However, increased soil pH significantly reduced the content of THSG and total anthraquinones. Reference-free transcriptome analysis was further conducted on P. multiflorum treated at pH 4.5 and 9.5, generating a total of 47,305 unigenes with an N50 of 2118 bp, of which 31,058 (65.65%) were annotated. Additionally, 2472 differentially expressed genes (DEGs) were identified. Among them, 17 DEGs associated with the biosynthesis of THSG and anthraquinones were screened. A comprehensive analysis of differential gene expression and effective component content demonstrated a significant positive correlation between the content of effective components and the 14 DEGs\' expression but a negative correlation with soil pH. This study highlighted the influence of varying soil pH values on the effective component content of P. multiflorum. Specific acidic conditions proved beneficial for the synthesis and accumulation of THSG and total anthraquinones in P. multiflorum, thereby enhancing the quality of the medicinal material.
摘要：
何首乌。是一种具有广泛分布和强大适应性的中药，但是目前缺乏对其耐酸碱性的全面研究。本研究旨在分析持续5个月的pH值胁迫对何首乌生理和光合参数的影响,和有效成分的含量。结果表明，pH胁迫显著影响了正常生长，生理功能，和光合指标。在土壤pH值为4.5时，多花假单胞菌的块茎表现出最高水平的2,3,5,4'-四羟基二苯乙烯-2-O-β-d-葡糖苷（THSG）和总蒽醌，分别为5.41％和0.38％，分别。然而,土壤pH值的增加显著降低了THSG和总蒽醌的含量。对在pH4.5和9.5下处理的多花假单胞菌进一步进行无参考转录组分析，产生总共47,305个单基因，N50为2118bp，其中注释了31,058（65.65%）。此外,鉴定了2472个差异表达基因(DEGs)。其中,筛选了17个与THSG和蒽醌生物合成相关的DEGs。对差异基因表达和有效成分含量的综合分析表明，有效成分含量与14个DEGs的表达呈显着正相关，而与土壤pH呈负相关。本研究强调了不同土壤pH值对多花紫苏有效成分含量的影响。特定的酸性条件被证明对多氟磷中THSG和总蒽醌的合成和积累有益，从而提高药材的质量。
