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Abstract：
Tamarindus indica L., is widely used tree in ayurvedic medicine. Here, we aimed to understand the presence of important constituents in seeds and peel of Tamarind fruits and their biological activities. Hence, seeds and peel of Tamarind fruits are used for further extraction process by soxhlet method (chloroform and ethyl acetate solvents). Results suggest that the ethyl acetate extract (seeds) consists of terpenoids (72.29 ± 0.513 mg/g), phenolic content (68.67 ± 2.11 mg/g) and flavonoids (26.36 ± 2.03 mg/g) whereas chloroform extract (seeds) has terpenoids (42.29 ± 0.98 mg/g). Similarly, chloroform extract (peel) has terpenoids (25.96 ± 3.20 mg/g) and flavonoids (46.36 ± 2.03 mg/g) whereas ethyl acetate extract (peel) has terpenoids (62.93 ± 0.987 mg/g). Furthermore, anti-inflammation activity results revealed that the chloroform extract of peel was found to be more effective with IC50 of 226.14 µg/ml by protein denaturation analysis and with IC50 of 245.5 µg/ml on lipoxygenase inhibition activity. Chloroform extract (peel and seeds) shown better antioxidant activity using DPPH than ethyl acetate extract (peel and seeds). Ethyl acetate extract of seeds showed impressive potency by inhibiting the growth of fungus, Candida albicans. Additionally, ethyl acetate extract of seeds showed impressive potency inhibiting the growth of Escherichia coli than Bacillus cereus. GC-MS analysis shown the existence of diverse set of phytochemicals in each extract. Overall, comparative studies highlight the effectiveness of seeds extracts than peel extracts. Moreover, GC-MS results suggest that the seeds and peel extracts (chloroform and ethyl acetate) contains a wide range of compounds (including flavonoids, isovanillic acid, fatty acids and phenolic compounds) which can be utilized for therapeutic purpose.
摘要：
罗曼玉，是阿育吠陀医学中广泛使用的树。这里,我们旨在了解罗望子果实种子和果皮中重要成分的存在及其生物学活性。因此，罗望子果实的种子和果皮通过索氏方法(氯仿和乙酸乙酯溶剂)用于进一步提取过程。结果表明，乙酸乙酯提取物（种子）由萜类化合物（72.29±0.513mg/g）组成，酚类含量（68.67±2.11mg/g）和类黄酮（26.36±2.03mg/g），而氯仿提取物（种子）具有萜类化合物（42.29±0.98mg/g）。同样，氯仿提取物（果皮）具有萜类化合物（25.96±3.20mg/g）和类黄酮（46.36±2.03mg/g），而乙酸乙酯提取物（果皮）具有萜类化合物（62.93±0.987mg/g）。此外，抗炎活性结果表明，通过蛋白质变性分析发现果皮氯仿提取物更有效，IC50为226.14µg/ml，对脂加氧酶抑制活性的IC50为245.5µg/ml。使用DPPH的氯仿提取物（果皮和种子）比乙酸乙酯提取物（果皮和种子）显示出更好的抗氧化活性。种子的乙酸乙酯提取物通过抑制真菌的生长显示出令人印象深刻的效力，白色念珠菌.此外,种子的乙酸乙酯提取物比蜡状芽孢杆菌显示出令人印象深刻的抑制大肠杆菌生长的效力。GC-MS分析显示每种提取物中存在不同组的植物化学物质。总的来说,比较研究强调了种子提取物比果皮提取物的有效性。此外，GC-MS结果表明，种子和果皮提取物（氯仿和乙酸乙酯）含有广泛的化合物（包括类黄酮，异香草酸，脂肪酸和酚类化合物）可用于治疗目的。
