Abstract：
OBJECTIVE: This study aimed to investigate miRNA expression profiles in individuals with periodontitis which is a chronic inflammatory condition affecting the integrity of the periodontal attachment. miRNAs play a crucial role in gene regulation through various mechanisms, making them potential diagnostic markers and therapeutic targets for various diseases.
METHODS: A total of 25 individuals with aggressive periodontitis and 25 controls were included in the study. Gingival tissues were collected for miRNA isolation and cDNA synthesis. miRNAs associated with periodontitis, including hsa-miR-185-5p, hsa-miR-17, hs-miR-146a, hs-miR-146b, hs-miR-155, hs-miR-203, hs-miR-205, hs-miR-223, and hsa-miR-21-3p, were analyzed using a combination of miRTarBase database analysis and literature mining was performed. Real-time PCR was used to assess the expression patterns of the target miRNAs, and the data were analyzed using the REST program.
RESULTS: The study revealed upregulated expression levels of hsa-miR-223-3p, hsa-miR-203b-5p, hsa-miR-146a-5p, hsa-miR-146b-5p, and hsa-miR-155-5p in individuals with periodontitis. Conversely, downregulated expression was observed for hsa-miR-185-5p, hsa-miR-21-3p, and hsa-miR-17-3p.
CONCLUSIONS: The findings suggest significant differences in the expression of specific miRNAs associated with inflammation in periodontitis. MZB1 acts as a hormone-regulated adipokine/pro-inflammatory cytokine, driving chronic inflammation and influencing cellular expansion. Predominantly expressed in marginal zone and B1 B cells, specialized subsets that respond rapidly to infections, MZB1 impacts immune protein synthesis and immune cell maturation, notably targeting microRNA-185 to potentially impede T cell development. Further research is needed to elucidate the functional significance and potential implications of these miRNAs.
CONCLUSIONS: miRNAs regulate the expression of target genes by finely tuning protein expression levels. The current findings provide compelling evidence of notable variations in the expression levels of specific miRNAs associated with inflammation in individuals affected by periodontitis; hence, miRNAs hold promise as potential therapeutic targets for periodontitis.
METHODS: A total of 25 individuals with aggressive periodontitis and 25 controls were included in the study. Gingival tissues were collected for miRNA isolation and cDNA synthesis. miRNAs associated with periodontitis, including hsa-miR-185-5p, hsa-miR-17, hs-miR-146a, hs-miR-146b, hs-miR-155, hs-miR-203, hs-miR-205, hs-miR-223, and hsa-miR-21-3p, were analyzed using a combination of miRTarBase database analysis and literature mining was performed. Real-time PCR was used to assess the expression patterns of the target miRNAs, and the data were analyzed using the REST program.
RESULTS: The study revealed upregulated expression levels of hsa-miR-223-3p, hsa-miR-203b-5p, hsa-miR-146a-5p, hsa-miR-146b-5p, and hsa-miR-155-5p in individuals with periodontitis. Conversely, downregulated expression was observed for hsa-miR-185-5p, hsa-miR-21-3p, and hsa-miR-17-3p.
CONCLUSIONS: The findings suggest significant differences in the expression of specific miRNAs associated with inflammation in periodontitis. MZB1 acts as a hormone-regulated adipokine/pro-inflammatory cytokine, driving chronic inflammation and influencing cellular expansion. Predominantly expressed in marginal zone and B1 B cells, specialized subsets that respond rapidly to infections, MZB1 impacts immune protein synthesis and immune cell maturation, notably targeting microRNA-185 to potentially impede T cell development. Further research is needed to elucidate the functional significance and potential implications of these miRNAs.
CONCLUSIONS: miRNAs regulate the expression of target genes by finely tuning protein expression levels. The current findings provide compelling evidence of notable variations in the expression levels of specific miRNAs associated with inflammation in individuals affected by periodontitis; hence, miRNAs hold promise as potential therapeutic targets for periodontitis.
摘要：
目的：本研究旨在研究牙周炎患者的miRNA表达谱，牙周炎是一种影响牙周附着完整性的慢性炎症状态。miRNAs通过各种机制在基因调控中起着至关重要的作用,使它们成为各种疾病的潜在诊断标志物和治疗靶标。
方法：本研究共纳入25名侵袭性牙周炎患者和25名对照者。收集牙龈组织用于miRNA分离和cDNA合成。与牙周炎相关的miRNAs，包括hsa-miR-185-5p,hsa-miR-17,hs-miR-146a,hs-miR-146b,hs-miR-155、hs-miR-203、hs-miR-205、hs-miR-223和hsa-miR-21-3p，使用miRTarBase数据库分析和文献挖掘相结合的方法进行分析。实时PCR用于评估靶miRNA的表达模式，并使用REST程序对数据进行分析。
结果：研究显示hsa-miR-223-3p的表达水平上调，hsa-miR-203b-5p,hsa-miR-146a-5p,hsa-miR-146b-5p,和hsa-miR-155-5p在牙周炎患者中的作用。相反，观察到hsa-miR-185-5p表达下调，hsa-miR-21-3p,和hsa-miR-17-3p.
结论：这些发现提示牙周炎中与炎症相关的特异性miRNAs的表达存在显著差异。MZB1作为激素调节的脂肪因子/促炎细胞因子，驱动慢性炎症和影响细胞扩增。在边缘区和B1B细胞中主要表达，对感染反应迅速的特殊子集，MZB1影响免疫蛋白合成和免疫细胞成熟，特别是靶向microRNA-185可能阻碍T细胞发育。需要进一步的研究来阐明这些miRNA的功能意义和潜在意义。
结论：miRNA通过微调蛋白质表达水平来调节靶基因的表达。目前的发现提供了令人信服的证据，表明在受牙周炎影响的个体中，与炎症相关的特定miRNA的表达水平存在显著变化；因此，miRNAs有望成为牙周炎的潜在治疗靶点。
方法：本研究共纳入25名侵袭性牙周炎患者和25名对照者。收集牙龈组织用于miRNA分离和cDNA合成。与牙周炎相关的miRNAs，包括hsa-miR-185-5p,hsa-miR-17,hs-miR-146a,hs-miR-146b,hs-miR-155、hs-miR-203、hs-miR-205、hs-miR-223和hsa-miR-21-3p，使用miRTarBase数据库分析和文献挖掘相结合的方法进行分析。实时PCR用于评估靶miRNA的表达模式，并使用REST程序对数据进行分析。
结果：研究显示hsa-miR-223-3p的表达水平上调，hsa-miR-203b-5p,hsa-miR-146a-5p,hsa-miR-146b-5p,和hsa-miR-155-5p在牙周炎患者中的作用。相反，观察到hsa-miR-185-5p表达下调，hsa-miR-21-3p,和hsa-miR-17-3p.
结论：这些发现提示牙周炎中与炎症相关的特异性miRNAs的表达存在显著差异。MZB1作为激素调节的脂肪因子/促炎细胞因子，驱动慢性炎症和影响细胞扩增。在边缘区和B1B细胞中主要表达，对感染反应迅速的特殊子集，MZB1影响免疫蛋白合成和免疫细胞成熟，特别是靶向microRNA-185可能阻碍T细胞发育。需要进一步的研究来阐明这些miRNA的功能意义和潜在意义。
结论：miRNA通过微调蛋白质表达水平来调节靶基因的表达。目前的发现提供了令人信服的证据，表明在受牙周炎影响的个体中，与炎症相关的特定miRNA的表达水平存在显著变化；因此，miRNAs有望成为牙周炎的潜在治疗靶点。
