Abstract：
We have performed an equine influenza (EI) serological study of the equine population in Algeria by testing 298 serum samples collected between February and August 2021 from 5 provinces. The results were obtained performing an NP-ELISA. Our results revealed that 49.3% (147/298) samples positive for antibodies to EI (H3N8). During this study and after a gap of one decade an outbreak of EI was reported in Algeria in the first week of March 2021. The disease was confirmed by virus detection from the nasal swabs (n = 39) by qRT-PCR and by identifying 5 EI seroconversion. The virus sequences were identified as H3N8 by sequencing the haemagglutinin (HA) and neuraminidase (NA) genes. Alignment of HA1 amino acid sequence confirmed that viruses belong to Clade 1 of the Florida sublineage in the American lineage. This study indicate the first detection of FC1 strain of EIV in Maghreb area.
摘要：
我们通过测试2021年2月至8月从5个省收集的298份血清样本，对阿尔及利亚的马种群进行了马流感（EI）血清学研究。通过NP-ELISA获得结果。我们的结果显示49.3%(147/298)样品对EI(H3N8)抗体呈阳性。在这项研究中，经过十年的间隔，在2021年3月的第一周，阿尔及利亚报告了EI的爆发。通过qRT-PCR从鼻拭子（n=39）进行病毒检测并鉴定5个EI血清转化来确认该疾病。通过测序血凝素(HA)和神经氨酸酶(NA)基因将病毒序列鉴定为H3N8。HA1氨基酸序列的比对证实病毒属于美国谱系中佛罗里达亚谱系的进化枝1。这项研究表明在马格里布地区首次检测到EIV的FC1菌株。
