PDF(Pubmed)
Abstract：
OBJECTIVE: In-depth studies on plant ion uptake and plant growth-promoting rhizobacteria (PGPR) at the molecular level will help to further reveal the effects of PGPR on plants and their interaction mechanisms under salt stress.
METHODS: Cotton was inoculated with a PGPR-Enterobacter cloacae Rs-35, and the ion uptake capacity, membrane transporter protein activity, and expression of key genes were determined under salt stress. Changes in the endogenous hormone content of cotton were also determined. Further, the genome-wide metabolic pathway annotation of E. cloacae Rs-35 and its differential enrichment pathway analysis of multi-omics under salinity environments were performed.
RESULTS: In a pot experiment of saline-alkali soil, E. cloacae Rs-35-treated cotton significantly increased its uptake of K+ and Ca2+ and decreased uptake of Na+, elevated the activity of the H+-ATPase, and increased the sensitivity of the Na+/H+ reverse transporter protein on the vesicle membrane. Meanwhile, inoculation with E. cloacae Rs-35 could promote cotton to maintain the indole-3-acetic acid (IAA) content under salt stress. Genome-wide annotation showed that E. cloacae Rs-35 was respectively annotated to 31, 38, and 130 related genes in osmotic stress, phytohormone and organic acid metabolism, and ion uptake metabolic pathway. Multi-omics differences analysis showed that E. cloacae Rs-35 were enriched to tryptophan metabolism, multiple amino acid biosynthesis, carbon and glucose synthesis, and oxidative phosphorylation metabolic pathways at the transcriptome, proteome, and metabolome.
CONCLUSIONS: E. cloacae Rs-35 can promote cotton balance cell ion concentration, stabilize intracellular IAA changes, stimulate induction of systemic tolerance, and promote the growth of cotton plants under salt stress.
METHODS: Cotton was inoculated with a PGPR-Enterobacter cloacae Rs-35, and the ion uptake capacity, membrane transporter protein activity, and expression of key genes were determined under salt stress. Changes in the endogenous hormone content of cotton were also determined. Further, the genome-wide metabolic pathway annotation of E. cloacae Rs-35 and its differential enrichment pathway analysis of multi-omics under salinity environments were performed.
RESULTS: In a pot experiment of saline-alkali soil, E. cloacae Rs-35-treated cotton significantly increased its uptake of K+ and Ca2+ and decreased uptake of Na+, elevated the activity of the H+-ATPase, and increased the sensitivity of the Na+/H+ reverse transporter protein on the vesicle membrane. Meanwhile, inoculation with E. cloacae Rs-35 could promote cotton to maintain the indole-3-acetic acid (IAA) content under salt stress. Genome-wide annotation showed that E. cloacae Rs-35 was respectively annotated to 31, 38, and 130 related genes in osmotic stress, phytohormone and organic acid metabolism, and ion uptake metabolic pathway. Multi-omics differences analysis showed that E. cloacae Rs-35 were enriched to tryptophan metabolism, multiple amino acid biosynthesis, carbon and glucose synthesis, and oxidative phosphorylation metabolic pathways at the transcriptome, proteome, and metabolome.
CONCLUSIONS: E. cloacae Rs-35 can promote cotton balance cell ion concentration, stabilize intracellular IAA changes, stimulate induction of systemic tolerance, and promote the growth of cotton plants under salt stress.
摘要：
目的：在分子水平上深入研究植物离子吸收和植物促生菌(PGPR),有助于进一步揭示盐胁迫下PGPR对植物的影响及其互作机制。
方法：用PGPR阴沟肠杆菌Rs-35接种棉花，膜转运蛋白活性，并确定了盐胁迫下关键基因的表达。还测定了棉花内源激素含量的变化。Further,在盐度环境下进行了阴沟肠球菌Rs-35的全基因组代谢途径注释及其多组学差异富集途径分析。
结果：在盐碱土的盆栽实验中，阴沟肠球菌Rs-35处理的棉花显着增加了其对K和Ca2的吸收，并减少了对Na的吸收，提高了H+-ATP酶的活性，并增加了囊泡膜上Na/H反向转运蛋白的敏感性。同时,在盐胁迫下，接种阴沟肠球菌Rs-35可以促进棉花维持吲哚-3-乙酸（IAA）含量。全基因组注释表明，阴沟肠杆菌Rs-35在渗透胁迫中分别注释了31、38和130个相关基因，植物激素和有机酸代谢，和离子摄取代谢途径。多组学差异分析显示阴沟肠球菌Rs-35对色氨酸代谢富集,多氨基酸生物合成，碳和葡萄糖的合成，和转录组的氧化磷酸化代谢途径，蛋白质组，和代谢组。
结论：E.泄殖腔Rs-35可以促进棉花平衡细胞离子浓度，稳定细胞内IAA变化，刺激全身耐受性的诱导，促进棉花在盐胁迫下的生长。
方法：用PGPR阴沟肠杆菌Rs-35接种棉花，膜转运蛋白活性，并确定了盐胁迫下关键基因的表达。还测定了棉花内源激素含量的变化。Further,在盐度环境下进行了阴沟肠球菌Rs-35的全基因组代谢途径注释及其多组学差异富集途径分析。
结果：在盐碱土的盆栽实验中，阴沟肠球菌Rs-35处理的棉花显着增加了其对K和Ca2的吸收，并减少了对Na的吸收，提高了H+-ATP酶的活性，并增加了囊泡膜上Na/H反向转运蛋白的敏感性。同时,在盐胁迫下，接种阴沟肠球菌Rs-35可以促进棉花维持吲哚-3-乙酸（IAA）含量。全基因组注释表明，阴沟肠杆菌Rs-35在渗透胁迫中分别注释了31、38和130个相关基因，植物激素和有机酸代谢，和离子摄取代谢途径。多组学差异分析显示阴沟肠球菌Rs-35对色氨酸代谢富集,多氨基酸生物合成，碳和葡萄糖的合成，和转录组的氧化磷酸化代谢途径，蛋白质组，和代谢组。
结论：E.泄殖腔Rs-35可以促进棉花平衡细胞离子浓度，稳定细胞内IAA变化，刺激全身耐受性的诱导，促进棉花在盐胁迫下的生长。
