Abstract：
The study investigated the chemical constituents from the whole herb of Carpesium cernuum. Three new diterpenoids were isolated from the whole herb of C. cernuum by column chromatography on silica gel, Sephadex LH-20, and semi-preparative HPLC. Their structures were identified by MS, NMR and other spectral techniques. The isolates were identified as(5Z)-2-oxo-2, 10, 14-trimethylhexadeca-5, 13-diene-11α, 18-diol(1),(2E, 10E)-7-[(acetyloxy)methyl]-3, 11, 15-trimethylhexadeca-2, 10, 14-triene-1, 12α-diol(2),(2E, 6Z)-3, 11, 15-trimethylhexadeca-2, 6, 14-triene-1, 12α, 19-triol(3), respectively. The cytotoxic activity of compounds 1-3 were investigated with DU-145, MCF-7, and A549 cells by MTT. The results showed that compound 1 and 3 had certain inhibitory effects on MCF-7 cells, with the inhibition rates of 45.06% and 29.40%, respectively.
摘要：
本研究调查了马尾花全草的化学成分。通过硅胶柱色谱从C.cernuum的整个草药中分离出三种新的二萜，SephadexLH-20和半制备型HPLC。它们的结构由MS鉴定，NMR和其他光谱技术。分离株被鉴定为（5Z）-2-氧代-2，10，14-三甲基十六烷基-5，13-二烯-11α，18-二醇(1)，(2E,10E)-7-[(乙酰氧基)甲基]-3,11,15-三甲基十六烷基-2,10,14-三烯-1,12α-二醇(2),(2E,6Z）-3，11，15-三甲基十六烷基-2，6，14-三烯-1，12α，19-三醇（3），分别。用DU-145、MCF-7和A549细胞通过MTT研究化合物1-3的细胞毒活性。结果表明,化合物1和3对MCF-7细胞有一定的抑制作用,抑制率分别为45.06%和29.40%,分别。
