Abstract：
In small and laboratory animals epididymal spermatozoa can be collected only by the mincing technique however, blood and cell debris contamination may adversely affect the quality of the samples. This study aimed at performing the retrograde flushing technique, for the first time, in the tomcat. In the first experiment attempts to insert a blunted needle into tomcats\' ductus deferentes were made and the cannulation time and the success rate were recorded. In the second experiment, insertion of the needle into the ductus was carried out under a stereomicroscope for one testis, while the other was submitted to mincing for sperm recovery and the samples obtained from paired testes were compared. In the first study, only twenty-eight percent of the cannulation attempts were successful whereas in the second experiment, 91% of the deferentes were successfully cannulated: 80% of the ducts were cannulated in less than three minutes. The stereomicroscope-aided retrograde flushing samples showed significantly higher sperm viability, motility and velocities, as compared to those resulted from mincing. The domestic cat serves as a model for more than 36 species of wild felids; the developed stereomicroscope-aided retrograde flushing technique has the potential to become the gold standard method for harvesting spermatozoa of endangered species that have a small ductus deferens.
摘要：
在小型和实验动物中，只能通过切碎技术收集附睾精子，血液和细胞碎片污染可能会对样品质量产生不利影响。这项研究旨在执行逆行冲洗技术，第一次,在tomcat。在第一个实验中，尝试将钝针插入tomcats导管输精管，并记录插管时间和成功率。在第二个实验中,将针头插入导管是在一个睾丸的立体显微镜下进行的，而另一个则接受切碎以进行精子回收，并比较了从配对睾丸中获得的样本。在第一项研究中，只有28%的插管尝试成功，而在第二次实验中，91％的输精管成功插管：80％的导管在不到三分钟的时间内插管。立体显微镜辅助的逆行冲洗样品显示出明显更高的精子活力，运动性和速度，与切碎产生的结果相比。家猫是超过36种野生猫科动物的模型；发达的立体显微镜辅助逆行冲洗技术有可能成为收获具有小输精管的濒危物种精子的黄金标准方法。
