关键词: Anaplasma capra Goat Phylogenetic analysis Türkiye Zoonoses gltA gene

Mesh : Animals Goats Goat Diseases / microbiology epidemiology Phylogeny Anaplasma / genetics isolation & purification Anaplasmosis / epidemiology microbiology Polymerase Chain Reaction / veterinary DNA, Bacterial / genetics

来  源:   DOI:10.1007/s11686-023-00758-y

Abstract:
BACKGROUND: A newly discovered zoonotic infection carried by ixodid ticks, Anaplasma capra, affects a wide variety of hosts, including numerous mammals. A. capra most likely infects erythrocytes or endothelial cells in mammals. This study aimed to investigate the A. capra pathogen in goats in Türkiye\'s Van province.
METHODS: A total of 200 goat blood samples were examined. Goat samples were subjected to partial amplification of the gltA gene fragment using a nested polymerase chain reaction.
RESULTS: A. capra DNA was detected in 0.5% of goat blood samples. Phylogenetic analysis of a partial gltA gene fragment showed that the Eastern Türkiye isolate, closely grouped with A. capra isolates reported from wild and domestic ruminants in France, Türkiye, and Kyrgyzstan, formed a distinct clade.
CONCLUSIONS: This is the first report of A. capra in goats in Van province, Eastern Türkiye.
摘要:
背景:一种新发现的人畜共患感染,由ixodid壁虱携带,无性体,影响各种各样的宿主,包括许多哺乳动物。A.capra最有可能感染哺乳动物的红细胞或内皮细胞。本研究旨在调查Türkiye'sVan省山羊中的A.capra病原体。
方法:共检查200份山羊血样。使用嵌套聚合酶链反应对山羊样品进行gltA基因片段的部分扩增。
结果:A.在0.5%的山羊血液样本中检测到capraDNA。部分gltA基因片段的系统发育分析表明,东部Türkiye分离株,与法国野生和家养反刍动物报告的卡普拉分离株紧密分类,蒂尔基耶,吉尔吉斯斯坦,形成了一个独特的进化枝。
结论:这是凡省山羊中A.capra的第一份报告,东蒂尔基耶。
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