PDF(Pubmed)
Abstract：
BACKGROUND: The synanthropic house fly (Musca domestica) can potentially contribute to the mechanical spread of eggs of Taenia and Ascaris spp. in the environment and between hosts. However, the absence of validated protocols to recover eggs hampers an in-depth analysis of the house fly\'s role in parasite egg transmission.
METHODS: The gastrointestinal tract and exoskeleton of euthanized house flies were spiked with Taenia saginata eggs. The performance of several recovery protocols, in terms of both the recovery rate and ease-of-use, was (microscopically) evaluated and compared. These protocols employed steps such as washing, maceration, filtration, flotation and both passive and centrifugal sedimentation. The final validated protocols were subsequently evaluated for the recovery of Ascaris suum eggs.
RESULTS: The final protocol validated for the recovery of T. saginata eggs from the house fly\'s gastrointestinal tract involved homogenization in phosphate-buffered saline and centrifugation at 2000 g for 2 min, yielding a recovery rate of 79.7%. This protocol required 6.5 min to perform (which included 1.5 min of hands-on time) and removed large debris particles that could hinder the differentiation of eggs from debris. Similarly, the final protocol validated for the recovery of T. saginata eggs from the fly\'s exoskeleton involved washing by vortexing for 2 min in Tween 80 (0.05%), 15 min of passive sedimentation and centrifugation at 2000 g for 2 min, yielding a recovery rate of 77.4%. This protocol required 20.5 min to perform (which included 3.5 min of hands-on time) and successfully removed debris. The same protocols yielded recovery rates of 74.2% and 91.5% for the recovery of A. suum eggs from the fly\'s gastrointestinal tract and exoskeleton, respectively.
CONCLUSIONS: Effective, simple and easy-to-use protocols were developed and validated for the recovery of T. saginata and A. suum eggs from the house fly\'s gastrointestinal tract and exoskeleton. These protocols can be applied to investigate the importance of flies as parasite egg transmitters in laboratory and field settings.
METHODS: The gastrointestinal tract and exoskeleton of euthanized house flies were spiked with Taenia saginata eggs. The performance of several recovery protocols, in terms of both the recovery rate and ease-of-use, was (microscopically) evaluated and compared. These protocols employed steps such as washing, maceration, filtration, flotation and both passive and centrifugal sedimentation. The final validated protocols were subsequently evaluated for the recovery of Ascaris suum eggs.
RESULTS: The final protocol validated for the recovery of T. saginata eggs from the house fly\'s gastrointestinal tract involved homogenization in phosphate-buffered saline and centrifugation at 2000 g for 2 min, yielding a recovery rate of 79.7%. This protocol required 6.5 min to perform (which included 1.5 min of hands-on time) and removed large debris particles that could hinder the differentiation of eggs from debris. Similarly, the final protocol validated for the recovery of T. saginata eggs from the fly\'s exoskeleton involved washing by vortexing for 2 min in Tween 80 (0.05%), 15 min of passive sedimentation and centrifugation at 2000 g for 2 min, yielding a recovery rate of 77.4%. This protocol required 20.5 min to perform (which included 3.5 min of hands-on time) and successfully removed debris. The same protocols yielded recovery rates of 74.2% and 91.5% for the recovery of A. suum eggs from the fly\'s gastrointestinal tract and exoskeleton, respectively.
CONCLUSIONS: Effective, simple and easy-to-use protocols were developed and validated for the recovery of T. saginata and A. suum eggs from the house fly\'s gastrointestinal tract and exoskeleton. These protocols can be applied to investigate the importance of flies as parasite egg transmitters in laboratory and field settings.
摘要：
背景：同人型家蝇（Muscadomestica）可能有助于the虫和A虫的卵的机械传播。在环境中和主机之间。然而,缺乏经过验证的回收卵子的协议阻碍了对家蝇在寄生虫卵传播中的作用的深入分析。
方法：在安乐死的家蝇的胃肠道和外骨骼中掺入牛带菌卵。几种恢复协议的性能，在回收率和易用性方面，（微观）评估和比较。这些协议采用的步骤，如洗涤，浸渍,过滤,浮选和被动和离心沉降。随后对最终验证的方案进行了评价，以恢复A虫卵。
结果：对从家蝇胃肠道中回收萨金纳塔虫卵的最终方案进行了验证，包括在磷酸盐缓冲盐水中进行匀浆，并在2000g离心2分钟，回收率为79.7%。该方案需要6.5分钟才能执行（包括1.5分钟的动手时间），并去除可能阻碍卵与碎片区分的大碎片颗粒。同样，最终方案验证了从果蝇的外骨骼中回收的萨金纳塔虫卵，包括通过在吐温80（0.05%）中涡旋2分钟进行洗涤，被动沉降15分钟，2000克离心2分钟，收率为77.4%。该方案需要20.5分钟才能执行（包括3.5分钟的动手时间）并成功清除碎片。同样的方案从苍蝇的胃肠道和外骨骼中回收A.suum卵的回收率分别为74.2%和91.5%。分别。
结论：有效，开发并验证了简单易用的方案，用于从家蝇的胃肠道和外骨骼中回收沙吉纳塔和沙乌姆卵。这些协议可用于研究苍蝇在实验室和野外环境中作为寄生虫卵发射器的重要性。
方法：在安乐死的家蝇的胃肠道和外骨骼中掺入牛带菌卵。几种恢复协议的性能，在回收率和易用性方面，（微观）评估和比较。这些协议采用的步骤，如洗涤，浸渍,过滤,浮选和被动和离心沉降。随后对最终验证的方案进行了评价，以恢复A虫卵。
结果：对从家蝇胃肠道中回收萨金纳塔虫卵的最终方案进行了验证，包括在磷酸盐缓冲盐水中进行匀浆，并在2000g离心2分钟，回收率为79.7%。该方案需要6.5分钟才能执行（包括1.5分钟的动手时间），并去除可能阻碍卵与碎片区分的大碎片颗粒。同样，最终方案验证了从果蝇的外骨骼中回收的萨金纳塔虫卵，包括通过在吐温80（0.05%）中涡旋2分钟进行洗涤，被动沉降15分钟，2000克离心2分钟，收率为77.4%。该方案需要20.5分钟才能执行（包括3.5分钟的动手时间）并成功清除碎片。同样的方案从苍蝇的胃肠道和外骨骼中回收A.suum卵的回收率分别为74.2%和91.5%。分别。
结论：有效，开发并验证了简单易用的方案，用于从家蝇的胃肠道和外骨骼中回收沙吉纳塔和沙乌姆卵。这些协议可用于研究苍蝇在实验室和野外环境中作为寄生虫卵发射器的重要性。
