Abstract:
<b>Background and Objective:</b> This plant is known in many parts of the world and is used as medicine in some countries. In Vietnam, people know how to use <i>Passiflora foetida </i>L.,<i> </i>as an herb. There are many research works in the world, most of them focus on medicinal properties. The objective of this study was to determine the genetic diversity of samples of <i>Passiflora foetida </i>L., yellow fruit based on agronomic traits and the gene region of \"<i>rbcL</i>\". <b>Materials and Methods:</b> Seed samples were arranged in a completely randomized design with three replications. The trial was a sample of <i>Passiflora foetida </i>L., species. The distance between sample plant is 3×2 m, so the total number of trees is 1,330 trees ha<sup>1</sup>. The genetic relationship between them was determined through the construction of a phylogenetic tree in the \"<i>rbcL</i>\" gene region sequence. Data analysis and processing methods done by using X software. <b>Results:</b> The PCR results that amplify the \"<i>rbcL</i>\" gene region around 670 bp, the PCR products were then sequenced. The sequence results were compared with the sequences on the NCBI gene bank, showing that the sequences of the nine varieties/species all coincided with the <i>Passiflora foetida </i>Sims species sequences with a high similarity coefficient from 98.77 to 99.9%. Genetically shows that all nine species samples belong to <i>Passiflora foetida</i> which can be classified into three genetic samples of An Giang (LA1), Ca Mau (LA2) and Can Tho (LA3) which are genetically close to each other, have the same index. <b>Conclusion:</b> The heritability in the broad sense is high in the two traits of stem height and leaf length, so it can be considered that these two traits are controlled by genes. The cultivation of algae seeds to improve the productivity of these medicinal plant varieties is important in providing raw materials for the oriental medicine industry.
摘要:
&lt;b&gt;背景和目标:&lt;/b&gt;这种植物在世界许多地方都是众所周知的,在某些国家被用作药物。在越南,人们知道如何使用<i>西番莲</i>L,<i></i>作为草药。世界上有许多研究工作,他们中的大多数专注于药用特性。本研究的目的是确定<i>Passiflorafoetida</i>L.,基于农艺性状和“<i>rbcL</i>\”的基因区域的黄色果实。<b>材料和方法:</b>种子样品以完全随机的设计排列,重复三次。试验是<i>西番莲</i>L.,种。样品间距离为3×2m,所以树的总数是1,330棵树ha<sup>1</sup>。通过构建“<i>rbcL</i>”基因区序列中的系统发育树,确定了它们之间的亲缘关系。利用X软件完成数据的分析和处理方法。<b>结果:</b>扩增670bp左右的\"<i>rbcL</i>\"基因区域的PCR结果,然后对PCR产物进行测序。将序列结果与NCBI基因库上的序列进行比较,表明这9个品种/种的序列均与&lt;i&gt;西番莲&lt;/i&gt;Sims物种序列重合,相似系数从98.77%到99.9%。遗传上表明,所有9个物种样本均属于&lt;i&gt;西番莲&lt;/i&gt;,可分为安江(LA1)的三个遗传样本,CaMau(LA2)和CanTho(LA3)在基因上彼此接近,有相同的指数。<b>结论:</b>茎高和叶长两个性状广义上的遗传力高,所以可以认为这两个性状是由基因控制的。培育藻类种子以提高这些药用植物品种的生产力对于为东方医药工业提供原料是重要的。