Abstract：
Biosurfactants are natural compounds with remarkable surface-active properties that may offer an eco-friendly alternative to conventional surfactants. Among them, mannosylerythritol lipids (MELs) stand out as an intriguing example of a glycolipid biosurfactant. MELs have been used in a variety of sectors for various applications, and are currently commercially produced. Industrially, they are used in the pharmaceutical, cosmetic, food, and agricultural industries, based on their ability to reduce surface tension and enhance emulsification. However, despite their utility, their production is comparatively limited industrially. From a bioprocessing standpoint, two areas of interest to improve the production process are upstream production and downstream (separation and purification) product recovery. The former has seen a significant amount of research, with researchers investigating several production factors: the microbial species or strain employed, the producing media composition, and the production strategy implemented. Improvement and optimization of these are key to scale-up the production of MELs. On the other hand, the latter has seen comparatively limited work presented in the literature. For the most part traditional separation techniques have been employed. This systematic review presents the production and purification methodologies used by researchers by comprehensively analyzing the current state-of-the-art with regards the production, separation, and purification of MELs. By doing so, the review presents different possible approaches, and highlights some potential areas for future work by identifying opportunities for the commercialization of MELs.
摘要：
生物表面活性剂是具有显著表面活性性质的天然化合物,其可以为常规表面活性剂提供生态友好的替代物。其中,甘露糖赤藓糖醇脂质（MEL）作为糖脂生物表面活性剂的一个有趣的例子。MEL已用于各种应用的各种部门，目前已商业化生产。在工业上,它们用于制药，化妆品,食物,和农业产业，基于它们降低表面张力和增强乳化作用的能力。然而,尽管它们很有用，他们的生产在工业上相对有限。从生物加工的角度来看，改善生产过程的两个感兴趣的领域是上游生产和下游(分离和纯化)产品回收。前者已经看到了大量的研究，研究人员调查了几种生产因素：所用的微生物物种或菌株，生产介质组成，以及实施的生产战略。这些的改进和优化是扩大MEL生产规模的关键。另一方面,后者在文献中提出的工作相对有限。大部分采用传统的分离技术。本系统综述通过全面分析当前最先进的生产和纯化方法，介绍了研究人员使用的生产和纯化方法，分离,和MEL的纯化。通过这样做,审查提出了不同的可能方法，并通过确定MEL商业化的机会，突出了一些未来工作的潜在领域。
