PDF(Pubmed)
Abstract：
Cellular reprogramming is characterized by the induced dedifferentiation of mature cells into a more plastic and potent state. This process can occur through artificial reprogramming manipulations in the laboratory such as nuclear reprogramming and induced pluripotent stem cell (iPSC) generation, and endogenously in vivo during amphibian limb regeneration. In amphibians such as the Mexican axolotl, a regeneration permissive environment is formed by nerve-dependent signaling in the wounded limb tissue. When exposed to these signals, limb connective tissue cells dedifferentiate into a limb progenitor-like state. This state allows the cells to acquire new pattern information, a property called positional plasticity. Here, we review our current understanding of endogenous reprogramming and why it is important for successful regeneration. We will also explore how naturally induced dedifferentiation and plasticity were leveraged to study how the missing pattern is established in the regenerating limb tissue.
摘要：
细胞重编程的特征在于成熟细胞被诱导去分化为更具可塑性和效力的状态。这个过程可以通过实验室中的人工重编程操作进行，例如核重编程和iPSC生成，并在两栖动物肢体再生过程中在体内内源性。在两栖动物中，例如墨西哥axolotl，再生许可环境是由受伤肢体组织中的神经依赖性信号形成的。当暴露于这些信号时，肢体结缔组织细胞去分化为肢体祖细胞样状态。这种状态允许细胞获取新的模式信息，一种叫做位置可塑性的性质。这里,我们回顾了我们目前对内源性重编程的理解，以及为什么它对成功再生很重要。我们还将探讨如何利用自然诱导的去分化和可塑性来研究如何在再生肢体组织中建立缺失模式。
