PDF(Pubmed)
Abstract：
BACKGROUND: Dry eyes and mouth are symptoms of Sjogren syndrome, which can occur on its own and be referred to as primary Sjogren syndrome or in conjunction with other rheumatic diseases like rheumatoid arthritis and be referred to as secondary Sjogren syndrome. Anti-muscarinic type 3 receptors have been linked to neurological issues as well as secretory dysfunction in Sjogren patients. Consequently, the purpose of this study is to determine the serum level of muscarinic acetylcholine receptor subtype 3 (m3AChR)-specific autoantibodies in rheumatoid arthritis (RA) patients and evaluate its relationship to disease activity, functional disability, and to study its role in the development of secondary Sjogren syndrome manifestations in those patients.
METHODS: In this cross-sectional study, 30 RA patients with secondary Sjogren syndrome signs and 30 RA patients without secondary Sjogren syndrome manifestations were included, along with 30 healthy volunteers who were aged, and sex matched as controls. All participants underwent thorough clinical examination, evaluation of disease activity using the DAS28 score, assessment of functional status using the modified health assessment questionnaire (MHAQ), and evaluation of the serum level of (m3AChR) by (ELISA).
RESULTS: When compared to RA patients without secondary Sjogren syndrome and healthy controls (20.09 ± 4.24, 18.36 ± 3.59 ng/ml respectively), the serum level of m3AChR antibodies among 30 RA patients with secondary Sjogren syndrome considerably increased (mean 25.98 ± 4.81 ng/ml).Analysis of the m3AChR\'s (ROC)-curve revealed that the three groups under study differed significantly (P < 0.001), with the AUC (0.806), cutoff (> 22.63ng/ml), sensitivity (73.33%), and specificity (86.67%) all exceeding the threshold. Additionally, there was a significant positive connection between the serum level of m3AChR and the following variables (P < 0.05): DAS scores, MHAQ score, number of tender & swollen joints, and acute phase reactants.
CONCLUSIONS: Autoantibodies against m3AChR may be one of the serum components involved in the pathophysiology of secondary Sjogren syndrome in RA patients, and because of their high sensitivity and specificity, they can be utilized as a diagnostic marker in these individuals.
METHODS: In this cross-sectional study, 30 RA patients with secondary Sjogren syndrome signs and 30 RA patients without secondary Sjogren syndrome manifestations were included, along with 30 healthy volunteers who were aged, and sex matched as controls. All participants underwent thorough clinical examination, evaluation of disease activity using the DAS28 score, assessment of functional status using the modified health assessment questionnaire (MHAQ), and evaluation of the serum level of (m3AChR) by (ELISA).
RESULTS: When compared to RA patients without secondary Sjogren syndrome and healthy controls (20.09 ± 4.24, 18.36 ± 3.59 ng/ml respectively), the serum level of m3AChR antibodies among 30 RA patients with secondary Sjogren syndrome considerably increased (mean 25.98 ± 4.81 ng/ml).Analysis of the m3AChR\'s (ROC)-curve revealed that the three groups under study differed significantly (P < 0.001), with the AUC (0.806), cutoff (> 22.63ng/ml), sensitivity (73.33%), and specificity (86.67%) all exceeding the threshold. Additionally, there was a significant positive connection between the serum level of m3AChR and the following variables (P < 0.05): DAS scores, MHAQ score, number of tender & swollen joints, and acute phase reactants.
CONCLUSIONS: Autoantibodies against m3AChR may be one of the serum components involved in the pathophysiology of secondary Sjogren syndrome in RA patients, and because of their high sensitivity and specificity, they can be utilized as a diagnostic marker in these individuals.
摘要：
背景：干燥的眼睛和口腔是干燥综合征的症状，它可以自己发生，被称为原发性干燥综合征，或与其他风湿性疾病如类风湿性关节炎结合，被称为继发性干燥综合征。抗毒蕈碱3型受体与干燥患者的神经系统问题以及分泌功能障碍有关。因此,这项研究的目的是确定类风湿关节炎（RA）患者中毒蕈碱乙酰胆碱受体亚型3（m3AChR）特异性自身抗体的血清水平，并评估其与疾病活动的关系，功能性残疾，并研究其在这些患者继发性干燥综合征表现发展中的作用。
方法：在这项横断面研究中，包括30例具有继发性干燥综合征体征的RA患者和30例无继发性干燥综合征表现的RA患者，以及30名健康的老年人志愿者，和性别匹配作为对照。所有参与者都接受了全面的临床检查，使用DAS28评分评估疾病活动性，使用改进的健康评估问卷(MHAQ)评估功能状态，并通过（ELISA）评估血清（m3AChR）的水平。
结果：与无继发性干燥综合征的RA患者和健康对照组相比（分别为20.09±4.24、18.36±3.59ng/ml），30例继发性干燥综合征RA患者血清m3AChR抗体水平显著升高(平均25.98±4.81ng/ml)。m3AChR（ROC）曲线分析显示，研究中的三组差异显着（P<0.001），与AUC（0.806），截止（>22.63ng/ml），灵敏度（73.33%），和特异性(86.67%)均超过阈值。此外,m3AChR的血清水平与以下变量之间存在显着正相关（P<0.05）：DAS评分，MHAQ得分,触痛和肿胀的关节数量，和急性期反应物。
结论：抗m3AChR的自身抗体可能是参与RA患者继发性干燥综合征病理生理的血清成分之一。由于它们的高灵敏度和特异性，它们可以用作这些个体的诊断标记。
方法：在这项横断面研究中，包括30例具有继发性干燥综合征体征的RA患者和30例无继发性干燥综合征表现的RA患者，以及30名健康的老年人志愿者，和性别匹配作为对照。所有参与者都接受了全面的临床检查，使用DAS28评分评估疾病活动性，使用改进的健康评估问卷(MHAQ)评估功能状态，并通过（ELISA）评估血清（m3AChR）的水平。
结果：与无继发性干燥综合征的RA患者和健康对照组相比（分别为20.09±4.24、18.36±3.59ng/ml），30例继发性干燥综合征RA患者血清m3AChR抗体水平显著升高(平均25.98±4.81ng/ml)。m3AChR（ROC）曲线分析显示，研究中的三组差异显着（P<0.001），与AUC（0.806），截止（>22.63ng/ml），灵敏度（73.33%），和特异性(86.67%)均超过阈值。此外,m3AChR的血清水平与以下变量之间存在显着正相关（P<0.05）：DAS评分，MHAQ得分,触痛和肿胀的关节数量，和急性期反应物。
结论：抗m3AChR的自身抗体可能是参与RA患者继发性干燥综合征病理生理的血清成分之一。由于它们的高灵敏度和特异性，它们可以用作这些个体的诊断标记。
