METHODS: In this cross-sectional study, 30 RA patients with secondary Sjogren syndrome signs and 30 RA patients without secondary Sjogren syndrome manifestations were included, along with 30 healthy volunteers who were aged, and sex matched as controls. All participants underwent thorough clinical examination, evaluation of disease activity using the DAS28 score, assessment of functional status using the modified health assessment questionnaire (MHAQ), and evaluation of the serum level of (m3AChR) by (ELISA).
RESULTS: When compared to RA patients without secondary Sjogren syndrome and healthy controls (20.09 ± 4.24, 18.36 ± 3.59 ng/ml respectively), the serum level of m3AChR antibodies among 30 RA patients with secondary Sjogren syndrome considerably increased (mean 25.98 ± 4.81 ng/ml).Analysis of the m3AChR\'s (ROC)-curve revealed that the three groups under study differed significantly (P < 0.001), with the AUC (0.806), cutoff (> 22.63ng/ml), sensitivity (73.33%), and specificity (86.67%) all exceeding the threshold. Additionally, there was a significant positive connection between the serum level of m3AChR and the following variables (P < 0.05): DAS scores, MHAQ score, number of tender & swollen joints, and acute phase reactants.
CONCLUSIONS: Autoantibodies against m3AChR may be one of the serum components involved in the pathophysiology of secondary Sjogren syndrome in RA patients, and because of their high sensitivity and specificity, they can be utilized as a diagnostic marker in these individuals.
方法:在这项横断面研究中,包括30例具有继发性干燥综合征体征的RA患者和30例无继发性干燥综合征表现的RA患者,以及30名健康的老年人志愿者,和性别匹配作为对照。所有参与者都接受了全面的临床检查,使用DAS28评分评估疾病活动性,使用改进的健康评估问卷(MHAQ)评估功能状态,并通过(ELISA)评估血清(m3AChR)的水平。
结果:与无继发性干燥综合征的RA患者和健康对照组相比(分别为20.09±4.24、18.36±3.59ng/ml),30例继发性干燥综合征RA患者血清m3AChR抗体水平显著升高(平均25.98±4.81ng/ml)。m3AChR(ROC)曲线分析显示,研究中的三组差异显着(P<0.001),与AUC(0.806),截止(>22.63ng/ml),灵敏度(73.33%),和特异性(86.67%)均超过阈值。此外,m3AChR的血清水平与以下变量之间存在显着正相关(P<0.05):DAS评分,MHAQ得分,触痛和肿胀的关节数量,和急性期反应物。
结论:抗m3AChR的自身抗体可能是参与RA患者继发性干燥综合征病理生理的血清成分之一。由于它们的高灵敏度和特异性,它们可以用作这些个体的诊断标记。