Abstract：
Eight previously undescribed compounds comprising pyrrole-2-carboxaldehyde derivatives, namely periplanpyrroles A-D (1-4), spirooxindole derivatives perispirooxindoles A (5) and B (6), and the phenolic compounds periplanetols G (7) and H (8), along with eight known compounds were isolated from the 70% ethanol extract of the whole bodies of Periplaneta americana. Their structures including absolute configurations were unambiguously identified by comprehensive spectroscopic analyses and computational methods. In addition, all compounds were evaluated for their activities against triple negative breast cancer in vitro. The wound healing assay revealed that 7, 9, and 11 significantly inhibit the migration of BT549 and MDA-MB-231 cells. Further observations made in Western blotting experiments showed that 7 could dose-dependently decrease the protein level of vimentin and N-cadherin in MDA-MB-231 and BT549 cells.
摘要：
包含吡咯-2-甲醛衍生物的八种先前未描述的化合物，即周围吡咯A-D(1-4)，螺羟吲哚衍生物前吡罗羟吲哚A（5）和B（6），和酚类化合物周围醇G(7)和H(8)，与八种已知的化合物一起从美洲大至今的整个身体的70％乙醇提取物中分离。通过全面的光谱分析和计算方法明确地确定了它们的结构，包括绝对构型。此外,在体外评价所有化合物对三阴性乳腺癌的活性.伤口愈合试验显示7、9和11显著抑制BT549和MDA-MB-231细胞的迁移。在Western印迹实验中进行的进一步观察表明，7可以剂量依赖性地降低MDA-MB-231和BT549细胞中波形蛋白和N-钙粘蛋白的蛋白水平。
