PDF(Pubmed)
Abstract：
The cell-to-cell communication primarily occurs through cell-surface and secreted proteins, which form a sophisticated network that coordinates systemic immune function. Uncovering these protein-protein interactions (PPIs) is indispensable for understanding the molecular mechanism and elucidating immune system aberrances under diseases. Traditional biological studies typically focus on a limited number of PPI pairs due to the relative low throughput of commonly used techniques. Encouragingly, classical methods have advanced, and many new systems tailored for large-scale protein-protein screening have been developed and successfully utilized. These high-throughput PPI investigation techniques have already made considerable achievements in mapping the immune cell interactome, enriching PPI databases and analysis tools, and discovering therapeutic targets for cancer and other diseases, which will definitely bring unprecedented insight into this field.
摘要：
细胞间的通讯主要通过细胞表面和分泌的蛋白质发生，它形成了一个协调全身免疫功能的复杂网络。揭示这些蛋白质-蛋白质相互作用（PPIs）对于理解疾病的分子机制和阐明免疫系统异常是必不可少的。由于常用技术的相对低的通量,传统的生物学研究通常集中于有限数量的PPI对。令人鼓舞的是，经典方法已经先进，许多为大规模蛋白质-蛋白质筛选量身定制的新系统已经开发并成功使用。这些高通量PPI研究技术已经在免疫细胞相互作用组的定位方面取得了相当大的成果,丰富PPI数据库和分析工具，发现癌症和其他疾病的治疗靶点，这肯定会给这个领域带来前所未有的洞察力。
