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Abstract：
UNASSIGNED: Yixishu lotion (YXSL) originates from the summary of traditional Chinese medicine clinical experience and constantly improves in practice in clinical validation of the exact efficacy of traditional Chinese medicine prescription. To explore the mechanism of YXSL in treating vaginitis and the potential mechanisms based on network pharmacology and experimental verification.
UNASSIGNED: The active components and drug-related targets of YXSL were retrieved from the TCMSP (Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform) database, and the target was predicted by the UniProt database. Searching for genes related to \'vaginitis\' disease in the GeneCards database, a total of 2581 drug targets were obtained. The interaction between proteins (PPI - protein-protein interaction) relationship was obtained by STRING database and visualized by Cytoscape software. Finally, the \'Bioconductor\' installation package in R software was used to analyze the GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways of the target.
UNASSIGNED: In this study, by the method of network pharmacology, the key active components of YXSL were flavonoids such as quercetin, apigenin, kaempferol, luteolin, β-sitosterol; the main core proteins included MAPK14, TP53, FGF2, ESR1, MAPK3, MAPK1, VEGFA, JUN, IL-6, and the KEGG pathway was mainly involved in MAPK pathway, Th17 pathway, Malaria, TNF pathway, and other signaling pathways. Animal experiments showed that the clinical symptoms and vaginal tissue lesions of the YXSL group and the fluconazole group were improved, and the levels of TNF-α (tumor necrosis factor alpha), IL-6 (interleukin-6), MDA (malondialdehyde), SOD (superoxide dismutase), IL-4, and IFN-γ (interferon-γ) in vaginal tissue and serum were better than the model group.
UNASSIGNED: YXSL may achieve its therapeutic effect on vaginitis by reducing the inflammatory response, improving oxidative stress response, and improving body immunity, and it provides a theoretical basis for further research on its pharmacodynamic material basis and mechanism of action.
UNASSIGNED: The active components and drug-related targets of YXSL were retrieved from the TCMSP (Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform) database, and the target was predicted by the UniProt database. Searching for genes related to \'vaginitis\' disease in the GeneCards database, a total of 2581 drug targets were obtained. The interaction between proteins (PPI - protein-protein interaction) relationship was obtained by STRING database and visualized by Cytoscape software. Finally, the \'Bioconductor\' installation package in R software was used to analyze the GO (Gene Ontology) and KEGG (Kyoto Encyclopedia of Genes and Genomes) pathways of the target.
UNASSIGNED: In this study, by the method of network pharmacology, the key active components of YXSL were flavonoids such as quercetin, apigenin, kaempferol, luteolin, β-sitosterol; the main core proteins included MAPK14, TP53, FGF2, ESR1, MAPK3, MAPK1, VEGFA, JUN, IL-6, and the KEGG pathway was mainly involved in MAPK pathway, Th17 pathway, Malaria, TNF pathway, and other signaling pathways. Animal experiments showed that the clinical symptoms and vaginal tissue lesions of the YXSL group and the fluconazole group were improved, and the levels of TNF-α (tumor necrosis factor alpha), IL-6 (interleukin-6), MDA (malondialdehyde), SOD (superoxide dismutase), IL-4, and IFN-γ (interferon-γ) in vaginal tissue and serum were better than the model group.
UNASSIGNED: YXSL may achieve its therapeutic effect on vaginitis by reducing the inflammatory response, improving oxidative stress response, and improving body immunity, and it provides a theoretical basis for further research on its pharmacodynamic material basis and mechanism of action.
摘要：
■益西舒洗剂（YXSL）源于对中医临床经验的总结，并在实践中不断完善，验证中医处方的确切疗效。基于网络药理学和实验验证，探讨YXSL治疗阴道炎的作用机制和潜在机制。
■从TCMSP（中药系统药理学数据库和分析平台）数据库中检索到YXSL的活性成分和药物相关靶标，目标由UniProt数据库预测。在GeneCards数据库中搜索与“阴道炎”疾病相关的基因，共获得2581个药物靶点。通过STRING数据库获得蛋白质之间的相互作用（PPI-蛋白质相互作用）关系，并通过Cytoscape软件进行可视化。最后,使用R软件中的“生物导体”安装包来分析目标的GO（基因本体论）和KEGG（京都基因和基因组百科全书）途径。
■在这项研究中，通过网络药理学的方法，YXSL的关键活性成分是黄酮类化合物，例如槲皮素，芹菜素,山奈酚,木犀草素,β-谷甾醇;主要核心蛋白包括MAPK14,TP53,FGF2,ESR1,MAPK3,MAPK1,VEGFA,JUN,IL-6和KEGG通路主要参与MAPK通路,Th17通路,疟疾,TNF通路,和其他信号通路。动物实验表明,YXSL组和氟康唑组的临床症状和阴道组织病变均得到改善,和TNF-α（肿瘤坏死因子α）的水平，IL-6(白介素-6),MDA（丙二醛），SOD（超氧化物歧化酶），阴道组织和血清中的IL-4和IFN-γ（干扰素-γ）均优于模型组。
■YXSL可能通过减少炎症反应来达到对阴道炎的治疗作用，改善氧化应激反应，提高身体免疫力，为进一步研究其药效物质基础和作用机制提供了理论依据。
■从TCMSP（中药系统药理学数据库和分析平台）数据库中检索到YXSL的活性成分和药物相关靶标，目标由UniProt数据库预测。在GeneCards数据库中搜索与“阴道炎”疾病相关的基因，共获得2581个药物靶点。通过STRING数据库获得蛋白质之间的相互作用（PPI-蛋白质相互作用）关系，并通过Cytoscape软件进行可视化。最后,使用R软件中的“生物导体”安装包来分析目标的GO（基因本体论）和KEGG（京都基因和基因组百科全书）途径。
■在这项研究中，通过网络药理学的方法，YXSL的关键活性成分是黄酮类化合物，例如槲皮素，芹菜素,山奈酚,木犀草素,β-谷甾醇;主要核心蛋白包括MAPK14,TP53,FGF2,ESR1,MAPK3,MAPK1,VEGFA,JUN,IL-6和KEGG通路主要参与MAPK通路,Th17通路,疟疾,TNF通路,和其他信号通路。动物实验表明,YXSL组和氟康唑组的临床症状和阴道组织病变均得到改善,和TNF-α（肿瘤坏死因子α）的水平，IL-6(白介素-6),MDA（丙二醛），SOD（超氧化物歧化酶），阴道组织和血清中的IL-4和IFN-γ（干扰素-γ）均优于模型组。
■YXSL可能通过减少炎症反应来达到对阴道炎的治疗作用，改善氧化应激反应，提高身体免疫力，为进一步研究其药效物质基础和作用机制提供了理论依据。
