PDF(Pubmed)
Abstract：
The nuclear factor-κB (NF-κB) transcription activation system involves disordered regions of both the NF-κB dimers and their inhibitors, the IκBs. The system is well-studied both at the cellular and biophysical levels affording a unique opportunity to compare and contrast the conclusions from both types of experiments. Through a combination of both experiments and theory, we have discovered that the RelA/p50 heterodimer and its inhibitor IκBα operate under kinetic control. Intrinsically disordered parts of both proteins are directly involved in temporal control and their folding and unfolding determines the rates of various processes. In this review, we show how the dynamic state of the intrinsically disordered sequences define the rates of intracellular processes.
摘要：
核因子-κB（NF-κB）转录激活系统涉及NF-κB二聚体及其抑制剂的无序区域，IκBs.该系统在细胞和生物物理水平上都得到了充分的研究，为比较和对比两种类型的实验的结论提供了独特的机会。通过实验和理论的结合，我们发现RelA/p50异二聚体及其抑制剂IκBα在动力学控制下起作用。两种蛋白质的内在无序部分直接参与时间控制，它们的折叠和展开决定了各种过程的速率。在这次审查中,我们展示了内在无序序列的动态状态如何定义细胞内过程的速率。
