PDF(Pubmed)
Abstract：
Articular cartilage exhibits a zonal architecture, comprising three distinct zones: superficial, middle, and deep. Collagen fibers, being the main solid constituent of articular cartilage, exhibit unique angular and size distribution in articular cartilage zones. There is a gap in knowledge on how the unique properties of collagen fibers across articular cartilage zones affect the scattering properties of the tissue.
This study hypothesizes that the structural properties of articular cartilage zones affect its scattering parameters. We provide scattering coefficient and scattering anisotropy factor of articular cartilage zones in the spectral band of 400 to 1400 nm. We enumerate the differences and similarities of the scattering properties of articular cartilage zones and provide reasoning for these observations.
We utilized collimated transmittance and integrating sphere measurements to estimate the scattering coefficients of bovine articular cartilage zones and bulk tissue. We used the relationship between the scattering coefficients to estimate the scattering anisotropy factor. Polarized light microscopy was applied to estimate the depth-wise angular distribution of collagen fibers in bovine articular cartilage.
We report that the Rayleigh scatterers contribution to the scattering coefficients, the intensity of the light scattered by the Rayleigh and Mie scatterers, and the angular distribution of collagen fibers across tissue depth are the key parameters that affect the scattering properties of articular cartilage zones and bulk tissue. Our results indicate that in the short visible region, the superficial and middle zones of articular cartilage affect the scattering properties of the tissue, whereas in the far visible and near-infrared regions, the articular cartilage deep zone determines articular cartilage scattering properties.
This study provides scattering properties of articular cartilage zones. Such findings support future research to utilize optical simulation to estimate the penetration depth, depth-origin, and pathlength of light in articular cartilage for optical diagnosis of the tissue.
This study hypothesizes that the structural properties of articular cartilage zones affect its scattering parameters. We provide scattering coefficient and scattering anisotropy factor of articular cartilage zones in the spectral band of 400 to 1400 nm. We enumerate the differences and similarities of the scattering properties of articular cartilage zones and provide reasoning for these observations.
We utilized collimated transmittance and integrating sphere measurements to estimate the scattering coefficients of bovine articular cartilage zones and bulk tissue. We used the relationship between the scattering coefficients to estimate the scattering anisotropy factor. Polarized light microscopy was applied to estimate the depth-wise angular distribution of collagen fibers in bovine articular cartilage.
We report that the Rayleigh scatterers contribution to the scattering coefficients, the intensity of the light scattered by the Rayleigh and Mie scatterers, and the angular distribution of collagen fibers across tissue depth are the key parameters that affect the scattering properties of articular cartilage zones and bulk tissue. Our results indicate that in the short visible region, the superficial and middle zones of articular cartilage affect the scattering properties of the tissue, whereas in the far visible and near-infrared regions, the articular cartilage deep zone determines articular cartilage scattering properties.
This study provides scattering properties of articular cartilage zones. Such findings support future research to utilize optical simulation to estimate the penetration depth, depth-origin, and pathlength of light in articular cartilage for optical diagnosis of the tissue.
摘要：
■关节软骨表现出带状结构，包括三个不同的区域：表面，中间,和深。胶原纤维,是关节软骨的主要固体成分，在关节软骨区表现出独特的角度和大小分布。关于跨关节软骨区的胶原纤维的独特性质如何影响组织的散射性质的知识存在差距。
■这项研究假设关节软骨区的结构特性会影响其散射参数。我们提供了400至1400nm光谱带中关节软骨区的散射系数和散射各向异性因子。我们列举了关节软骨区散射特性的差异和相似性，并为这些观察结果提供了推理。
■我们利用准直透射率和积分球测量来估计牛关节软骨区和块状组织的散射系数。我们使用散射系数之间的关系来估计散射各向异性因子。应用偏振光显微镜评估牛关节软骨中胶原纤维的深度角度分布。
■我们报告说，瑞利散射体对散射系数的贡献，由Rayleigh和Mie散射体散射的光的强度,胶原纤维在组织深度上的角度分布是影响关节软骨区和块状组织散射特性的关键参数。我们的结果表明，在短可见区域，关节软骨的浅表和中间区影响组织的散射特性，而在远视和近红外区域，关节软骨深部区决定了关节软骨的散射特性。
■本研究提供了关节软骨区的散射特性。这些发现支持未来的研究利用光学模拟来估计穿透深度，深度起源,和关节软骨中的光程长度，用于组织的光学诊断。
■这项研究假设关节软骨区的结构特性会影响其散射参数。我们提供了400至1400nm光谱带中关节软骨区的散射系数和散射各向异性因子。我们列举了关节软骨区散射特性的差异和相似性，并为这些观察结果提供了推理。
■我们利用准直透射率和积分球测量来估计牛关节软骨区和块状组织的散射系数。我们使用散射系数之间的关系来估计散射各向异性因子。应用偏振光显微镜评估牛关节软骨中胶原纤维的深度角度分布。
■我们报告说，瑞利散射体对散射系数的贡献，由Rayleigh和Mie散射体散射的光的强度,胶原纤维在组织深度上的角度分布是影响关节软骨区和块状组织散射特性的关键参数。我们的结果表明，在短可见区域，关节软骨的浅表和中间区影响组织的散射特性，而在远视和近红外区域，关节软骨深部区决定了关节软骨的散射特性。
■本研究提供了关节软骨区的散射特性。这些发现支持未来的研究利用光学模拟来估计穿透深度，深度起源,和关节软骨中的光程长度，用于组织的光学诊断。
