Abstract：
Osteosarcoma, the most common primary bone cancer that affects adolescents worldwide, has the early metastatic potential to be responsible for high mortality rates. Morin has a multipurpose role in numerous cancers, whereas little is known about its role in osteosarcoma migration and invasion. Therefore, we hypothesized that morin suppresses the invasive activities and the migratory potential of human osteosarcoma cells. Our results showed that morin reduced migration and invasion capabilities in human osteosarcoma U2OS and HOS cells. Moreover, morin inhibited the urokinase plasminogen activator (uPA) expression through a signal transducer and an activator of transcription-3 (STAT3) phosphorylation. After STAT3 overexpression, the decrease of the migratory potential and uPA expression caused by 100 μM of morin in U2OS cells was countered, indicating that STAT3 contributes to the antimetastatic property of morin in human osteosarcoma cells by reducing uPA. In conclusion, morin may be a potential candidate for the antimetastatic treatment of human osteosarcoma.
摘要：
骨肉瘤,影响全球青少年的最常见的原发性骨癌，具有导致高死亡率的早期转移潜力。莫林在许多癌症中具有多用途作用，而对其在骨肉瘤迁移和侵袭中的作用知之甚少。因此,我们假设morin抑制人骨肉瘤细胞的侵袭活动和迁移潜力。我们的结果表明，sorin降低了人骨肉瘤U2OS和HOS细胞的迁移和侵袭能力。此外，莫林通过信号转导和转录激活因子3（STAT3）磷酸化抑制尿激酶纤溶酶原激活因子（uPA）的表达。STAT3过表达后，U2OS细胞中100μMmorin引起的迁移潜力和uPA表达的降低被抵消，表明STAT3通过减少uPA有助于人骨肉瘤细胞中桑蛋白的抗转移特性。总之,莫林可能是人类骨肉瘤抗转移治疗的潜在候选者。
