Abstract：
In recent years, there has been a notable increase in interest surrounding nanozymes due to their ability to imitate the functions and address the limitations of natural enzymes. The scientific community has been greatly intrigued by the study of nanoceria, primarily because of their distinctive physicochemical characteristics, which include a variety of enzyme-like activities, affordability, exceptional stability, and the ability to easily modify their surfaces. Consequently, nanoceria have found extensive use in various biosensing applications. However, the impact of its redox activity on the enzymatic catalytic mechanism remains a subject of debate, as conflicting findings in the literature have presented both pro-oxidant and antioxidant effects. Herein, we creatively propose a seesaw model to clarify the regulatory mechanism on redox balance and survey possible mechanisms of multienzyme mimetic properties of nanoceria. In addition, this review aims to showcase the latest advancements in this field by systematically discussing over 180 research articles elucidating the significance of ceria-based nanozymes in enhancing, downsizing, and enhancing the efficacy of point-of-care (POC) diagnostics. These advancements align with the ASSURED criteria established by the World Health Organization (WHO). Furthermore, this review also examines potential constraints in order to offer readers a concise overview of the emerging role of nanoceria in the advancement of POC diagnostic systems for future biosensing applications.
摘要：
近年来,由于纳米酶能够模仿功能并解决天然酶的局限性，因此人们对纳米酶的兴趣显着增加。科学界对纳米二氧化硅的研究非常感兴趣，主要是因为它们独特的物理化学特征，其中包括各种类似酶的活性，负担能力，异常稳定，以及轻松修改其表面的能力。因此,在各种生物传感应用中发现了纳米二氧化铈的广泛用途。然而,其氧化还原活性对酶催化机理的影响仍然是一个争论的话题，由于文献中相互矛盾的发现提出了促氧化剂和抗氧化剂的作用。在这里，我们创造性地提出了一个跷跷板模型，以阐明氧化还原平衡的调节机制，并探讨纳米二氧化铈多酶模拟特性的可能机制。此外,这篇综述旨在通过系统地讨论180多篇研究文章来展示该领域的最新进展，阐明基于二氧化铈的纳米酶在增强，缩小规模，并提高现场护理（POC）诊断的疗效。这些进步符合世界卫生组织（WHO）制定的ASSURED标准。此外，这篇综述还研究了潜在的制约因素，以便为读者提供纳米二氧化硅在POC诊断系统的未来生物传感应用中的新兴作用的简要概述。
