Abstract：
Erythritol (ERT) and L-ascorbyl-2-phosphate (APS) are bacteriostatic, but their effects on staphylococcal skin infections remain unknown. We aimed to determine whether ERT combined with APS inhibits the growth of staphylococci that are commonly isolated from pyoderma skin lesions in dogs. We investigated the individual and combined effects of ERT and APS on the growth of Staphylococcus pseudintermedius, S. schleiferi, and S. aureus using turbidity assays in vitro. Skin lesions from 10 dogs with superficial pyoderma were topically treated with 5% ERT and 0.1% APS for 28 days, and swabbed skin samples were then analyzed using 16S rRNA amplicon sequencing and quantitative real-time PCR (qPCR). Results showed that ERT inhibited S. pseudintermedius growth regardless of harboring the mecA gene, and APS increased the inhibitory effects of ERT against S. pseudintermedius, S. schleiferi, and S. aureus in vitro. Moreover, combined ERT and APS decreased the prevalence of staphylococci on canine skin lesions at the genus level. The combination slightly increased the α-diversity but did not affect the β-diversity of the microbiota. The qPCR results revealed that the combination significantly decreased S. pseudintermedius and S. schleiferi in skin lesions. Topical administration of EPS combined with APS can prevent staphylococcal colonization on the surface of mammalian skin. The results of this study may provide an alternative to systemic antibiotics for treating superficial pyoderma on mammalian skin surfaces.
摘要：
赤藓糖醇（ERT）和L-抗坏血酸-2-磷酸酯（APS）是抑菌，但它们对葡萄球菌皮肤感染的影响尚不清楚。我们旨在确定ERT与APS联合是否会抑制通常从狗脓皮病皮肤病变中分离出的葡萄球菌的生长。我们调查了ERT和APS对假中介葡萄球菌生长的个体和联合作用，S.Schleiferi,和金黄色葡萄球菌使用体外浊度测定。用5%ERT和0.1%APS局部治疗10只浅表脓皮病犬的皮肤病变28天，然后使用16SrRNA扩增子测序和定量实时PCR(qPCR)分析擦拭的皮肤样品。结果表明，无论携带mecA基因，ERT都能抑制假中介杆菌的生长，APS增加了ERT对假中介链球菌的抑制作用，S.Schleiferi,和金黄色葡萄球菌在体外。此外，联合ERT和APS在属水平上降低了葡萄球菌在犬皮肤病变上的患病率.该组合略微增加了微生物群的α-多样性，但不影响微生物群的β-多样性。qPCR结果表明，该组合显着降低了皮肤病变中的假中间链球菌和S.schleiferi。EPS联合APS的局部给药可以防止葡萄球菌在哺乳动物皮肤表面的定植。这项研究的结果可能为全身性抗生素提供一种替代方法，用于治疗哺乳动物皮肤表面的浅层脓皮病。
