Abstract：
Oriental theileriosis caused by Theileria orientalis, previously considered a benign disease, is posing a significant threat to the livestock industry across the globe. To elucidate the prevalence of Theileria orientalis in ticks and their host, the Mithun, a comprehensive study was undertaken in the two northeastern states of India, viz. Nagaland and Arunachal Pradesh. A total of 340 of Rhipicephalus microplus ticks and 25 Ambylomma sp. ticks were screened for the presence of Theileria orientalis through PCR. Among the R. microplus ticks examined, 25 of them tested positive for T. orientalis infection whereas none of the Amblyomma ticks was positive. Additionally, a total of 275 blood samples were collected from Mithun from Arunachal and Nagaland and 31 animals were found to be positive for T. orientalis infection. Notably, six positive cases were identified in Porba (Phek district), six in Tening, and one in Bamsiakilwa village (Peren district) of Nagaland. Moreover, out of the 41 animals examined at Medziphema farms, Nagaland, 18 were found to be positive for T. orientalis infection. Moreover, the phylogenetic investigation has unveiled the presence of the highly pathogenic Type 2 (Ikeda) T. orientalis genotype in Mithun, supported by a strong bootstrap value of 100%. This study marks the initial documentation of oriental theileriosis in mithun. It underscores the need for vigilant monitoring and active surveillance of mithun populations in the northeastern states of India. Timely treatment of infected animals is imperative to avert economic losses for the farmers.
摘要：
东方TheileriaOrientalis引起的东方theileriosis，以前被认为是良性疾病，对全球畜牧业构成重大威胁。为了阐明壁虱及其宿主中东方Theileria的患病率，Mithun,在印度东北部的两个州进行了一项全面的研究，viz.那加兰邦和阿鲁纳恰尔邦。总共有340个小rypicephalusplustick和25个Ambylommasp。通过PCR筛选壁虱的存在。在检查的R.microplus蜱中，其中25例测试为东方T.g感染呈阳性，而无一个是阳性。此外,从Arunachal和Nagaland的Mithun收集了总共275份血液样本，发现31只动物对T.Overentalis感染呈阳性。值得注意的是,在Porba（Phek区）发现了6例阳性病例，六个在泰宁，和一个在那加兰邦的Bamsiakilwa村（佩伦区）。此外，在Medziphema农场检查的41只动物中，那加兰,发现18例东方毛虫感染呈阳性。此外，系统发育研究揭示了Mithun中存在高致病性2型（Ikeda）T.Orientalis基因型，由100%的强引导值支持。这项研究标志着mithun东方theileriosis的初步文献。它强调了对印度东北部各州的mithun人口进行警惕和积极监视的必要性。及时治疗受感染的动物对于避免农民的经济损失至关重要。
