PDF(Pubmed)
Abstract：
Microcrystal electron diffraction, commonly referred to as MicroED, has become a powerful tool for high-resolution structure determination. The method makes use of cryogenic transmission electron microscopes to collect electron diffraction data from crystals that are several orders of magnitude smaller than those used by other conventional diffraction techniques. MicroED has been used on a variety of samples including soluble proteins, membrane proteins, small organic molecules, and materials. Here we will review the MicroED method and highlight recent advancements to the methodology, as well as describe applications of MicroED within the fields of structural biology and chemical crystallography.
摘要：
微晶电子衍射，通常被称为MicroED，已成为高分辨率结构确定的有力工具。该方法利用低温透射电子显微镜从晶体中收集电子衍射数据，该数据比其他常规衍射技术所用的数据小几个数量级。MicroED已用于各种样品，包括可溶性蛋白质，膜蛋白，有机小分子,和材料。在这里，我们将回顾MicroED方法，并强调该方法的最新进展，以及描述MicroED在结构生物学和化学晶体学领域中的应用。
