Abstract：
UNASSIGNED: Microscopic examination of cells and tissues requires the preparation of very thin and good-quality sections mounted on glass slides and appropriately stained to demonstrate normal and abnormal structures. Before this step, the tissue must undergo preparatory treatment known as tissue processing. The various stages of tissue processing are dehydration, clearing, impregnation, and embedding, each with a particular duration for proper completion of the process. Xylene is the most frequently used clearing agent whose carcinogenic potential is well documented. Hence, attempts were made to substitute xylene with a biosafe clearing agent. The present study aimed to evaluate and compare the efficacy of hematoxylin and eosin stain (H and E stain) when xylene is completely replaced by turpentine or kerosene oil.
UNASSIGNED: A total number of 50 tissue samples were taken in the study, which included 40 study samples and 10 controls. All the samples were randomly separated into three groups and routine tissue processing and H and E staining were performed. The result was further subjected to statistical analysis by using Fisher\'s exact test. Group-1: Ten tissue samples were processed and H and E staining was done in xylene. Group-2: Twenty tissue samples were processed and H and E staining was done in turpentine oil. Group-3: Twenty tissue samples were processed and H and E staining was done in kerosene oil.
UNASSIGNED: Nuclear staining, cell morphology, and uniformity of staining were better in kerosene sections, while cytoplasmic and clarity of staining of turpentine sections were comparable with xylene sections.
UNASSIGNED: Turpentine and kerosene as clearing agents can be used in the future with certain modifications in their concentration and routine staining protocol.
UNASSIGNED: A total number of 50 tissue samples were taken in the study, which included 40 study samples and 10 controls. All the samples were randomly separated into three groups and routine tissue processing and H and E staining were performed. The result was further subjected to statistical analysis by using Fisher\'s exact test. Group-1: Ten tissue samples were processed and H and E staining was done in xylene. Group-2: Twenty tissue samples were processed and H and E staining was done in turpentine oil. Group-3: Twenty tissue samples were processed and H and E staining was done in kerosene oil.
UNASSIGNED: Nuclear staining, cell morphology, and uniformity of staining were better in kerosene sections, while cytoplasmic and clarity of staining of turpentine sections were comparable with xylene sections.
UNASSIGNED: Turpentine and kerosene as clearing agents can be used in the future with certain modifications in their concentration and routine staining protocol.
摘要：
■对细胞和组织进行显微镜检查需要制备非常薄且质量好的切片，这些切片安装在载玻片上并进行适当的染色以显示正常和异常的结构。在这一步之前，组织必须经历称为组织处理的预备处理。组织处理的各个阶段是脱水，清除,浸渍,和嵌入，每个都有一个特定的持续时间，以正确完成该过程。二甲苯是最常用的清洁剂，其致癌潜力已得到充分证明。因此，尝试用生物安全的清除剂代替二甲苯。本研究旨在评估和比较当二甲苯完全被松节油或煤油代替时苏木精和曙红染色（H和E染色）的功效。
■本研究共采集了50个组织样本，其中包括40个研究样本和10个对照。将所有样品随机分为3组,并进行常规组织处理和H和E染色。使用Fisher精确检验进一步对结果进行统计分析。组1:处理10个组织样品并在二甲苯中进行H和E染色。组2:处理20个组织样品并在松节油中进行H和E染色。组3:处理20个组织样品并在煤油中进行H和E染色。
■核染色，细胞形态学,煤油切片染色均匀性较好，而松节油切片的细胞质和染色的透明度与二甲苯切片相当。
■松节油和煤油作为清除剂可以在将来使用，但对其浓度和常规染色方案进行某些修改。
■本研究共采集了50个组织样本，其中包括40个研究样本和10个对照。将所有样品随机分为3组,并进行常规组织处理和H和E染色。使用Fisher精确检验进一步对结果进行统计分析。组1:处理10个组织样品并在二甲苯中进行H和E染色。组2:处理20个组织样品并在松节油中进行H和E染色。组3:处理20个组织样品并在煤油中进行H和E染色。
■核染色，细胞形态学,煤油切片染色均匀性较好，而松节油切片的细胞质和染色的透明度与二甲苯切片相当。
■松节油和煤油作为清除剂可以在将来使用，但对其浓度和常规染色方案进行某些修改。
