UNASSIGNED: A total number of 50 tissue samples were taken in the study, which included 40 study samples and 10 controls. All the samples were randomly separated into three groups and routine tissue processing and H and E staining were performed. The result was further subjected to statistical analysis by using Fisher\'s exact test. Group-1: Ten tissue samples were processed and H and E staining was done in xylene. Group-2: Twenty tissue samples were processed and H and E staining was done in turpentine oil. Group-3: Twenty tissue samples were processed and H and E staining was done in kerosene oil.
UNASSIGNED: Nuclear staining, cell morphology, and uniformity of staining were better in kerosene sections, while cytoplasmic and clarity of staining of turpentine sections were comparable with xylene sections.
UNASSIGNED: Turpentine and kerosene as clearing agents can be used in the future with certain modifications in their concentration and routine staining protocol.
■本研究共采集了50个组织样本,其中包括40个研究样本和10个对照。将所有样品随机分为3组,并进行常规组织处理和H和E染色。使用Fisher精确检验进一步对结果进行统计分析。组1:处理10个组织样品并在二甲苯中进行H和E染色。组2:处理20个组织样品并在松节油中进行H和E染色。组3:处理20个组织样品并在煤油中进行H和E染色。
■核染色,细胞形态学,煤油切片染色均匀性较好,而松节油切片的细胞质和染色的透明度与二甲苯切片相当。
■松节油和煤油作为清除剂可以在将来使用,但对其浓度和常规染色方案进行某些修改。