PDF(Pubmed)
Abstract：
Biodesulfurization (BD) systems that treat sour gas employ mixtures of haloalkaliphilic sulfur-oxidizing bacteria to convert sulfide to elemental sulfur. In the past years, these systems have seen major technical innovations that have led to changes in microbial community composition. Different studies have identified and discussed the microbial communities in both traditional and improved systems. However, these studies do not identify metabolically active community members and merely focus on members\' presence/absence. Therefore, their results cannot confirm the activity and role of certain bacteria in the BD system. To investigate the active community members, we determined the microbial communities of six different runs of a pilot-scale BD system. 16S rRNA gene-based amplicon sequencing was performed using both DNA and RNA. A comparison of the DNA- and RNA-based sequencing results identified the active microbes in the BD system. Statistical analyses indicated that not all the existing microbes were actively involved in the system and that microbial communities continuously evolved during the operation. At the end of the run, strains affiliated with Alkalilimnicola ehrlichii and Thioalkalivibrio sulfidiphilus were confirmed as the most active key bacteria in the BD system. This study determined that microbial communities were shaped predominantly by the combination of hydraulic retention time (HRT) and sulfide concentration in the anoxic reactor and, to a lesser extent, by other operational parameters.IMPORTANCEHaloalkaliphilic sulfur-oxidizing bacteria are integral to biodesulfurization (BD) systems and are responsible for converting sulfide to sulfur. To understand the cause of conversions occurring in the BD systems, knowing which bacteria are present and active in the systems is essential. So far, only a few studies have investigated the BD system\'s microbial composition, but none have identified the active microbial community. Here, we reveal the metabolically active community, their succession, and their influence on product formation.
摘要：
处理酸性气体的生物脱硫(BD)系统采用亲卤碱性硫氧化细菌的混合物将硫化物转化为元素硫。在过去的几年里,这些系统已经看到了重大的技术创新，导致微生物群落组成的变化。不同的研究已经确定并讨论了传统和改进系统中的微生物群落。然而,这些研究没有确定代谢活跃的社区成员，而仅仅关注成员的存在/不存在。因此,他们的结果不能证实某些细菌在BD系统中的活性和作用。为了调查活跃的社区成员，我们确定了中试规模BD系统的六个不同运行的微生物群落。使用DNA和RNA进行基于16SrRNA基因的扩增子测序。基于DNA和RNA的测序结果的比较鉴定了BD系统中的活性微生物。统计分析表明，并非所有现有的微生物都积极参与该系统，并且在操作过程中微生物群落不断进化。在跑步结束时，与Alkalilimnicolaehrlichii和硫碱性弧菌相关的菌株被确认为BD系统中最活跃的关键细菌。这项研究确定，微生物群落的形状主要由水力停留时间（HRT）和硫化物浓度的组合在缺氧反应器中，在较小程度上，其他操作参数。重要的嗜碱性硫氧化细菌是生物脱硫（BD）系统的组成部分，负责将硫化物转化为硫。要了解BD系统中发生转换的原因，知道哪些细菌在系统中存在和活跃是必不可少的。到目前为止,只有少数研究调查了BD系统的微生物组成，但是没有人发现活跃的微生物群落。这里,我们揭示了代谢活跃的社区，他们的继承,以及它们对产品形成的影响。
