Here, we attempted to determine the activation of innate immune responses in the lungs of mice inoculated intranasally with BAd-H5HA compared to the HAd-H5HA-inoculated group.
RNA-Seq analyses of the lung tissues revealed differential expression (DE) of genes involved in innate and adaptive immunity in animals immunized with BAd-H5HA. The top ten enhanced genes were verified by RT-PCR. Consistently, there were transient increases in the levels of cytokines (IL-1α, IL-1β, IL-5, TNF- α, LIF, IL-17, G-CSF, MIP-1β, MCP-1, MIP-2, and GM-CSF) and toll-like receptors in the lungs of the group inoculated with BAdV vectors compared to that of the HAdV vector group.
These results demonstrate that the BAdV vectors induce enhanced innate and adaptive immunity-related factors compared to HAdV vectors in mice. Thus, the BAdV vector platform could be an excellent gene delivery system for recombinant vaccines and cancer immunotherapy.
■这里,我们试图确定与HAd-H5HA接种组相比,鼻内接种BAd-H5HA的小鼠肺中先天免疫应答的激活。
■肺组织的RNA-Seq分析揭示了在用BAd-H5HA免疫的动物中参与先天和适应性免疫的基因的差异表达(DE)。通过RT-PCR验证前10个增强基因。始终如一,细胞因子(IL-1α,IL-1β,IL-5,TNF-α,LIF,IL-17,G-CSF,MIP-1β,与HAdV载体组相比,接种BAdV载体组的肺中的MCP-1,MIP-2和GM-CSF)和toll样受体。
■这些结果表明,与HAdV载体相比,BAdV载体在小鼠中诱导增强的先天和适应性免疫相关因子。因此,BAdV载体平台可能是重组疫苗和癌症免疫治疗的优良基因传递系统。