PDF(Pubmed)
Abstract：
Deepor Beel, located in the state of Assam in India, is a Wetland of International Importance with a Wildlife Sanctuary and is the only RAMSAR site in the state. Though of invaluable ecological significance, the wetland is facing anthropogenic stressors, leading to rapid degradation of ecological health. In December 2022, surface water was collected from six stations of Deepor Beel to elucidate biological communities using the eDNA approach. At the time of sampling, in-situ environmental parameters were measured in triplicates. The dissolved nutrients and concentrations of metals and metalloids were estimated using UV-Vis Spectrophotometry and ICP-MS approaches respectively. The study revealed a high concentration of dissolved nitrate in the surface water. High-throughput sequencing using Nanopore sequencing chemistry in a MinION platform indicated the overwhelming abundance of Moraxellaceae (Prokaryotes) and Eumetazoa (Eukaryotes). The abundance of Cyprinidae were also encountered in the studied wetland reflecting the biodiversity of fish populations. High nitrate along with elucidated microbial signals are crucial to designate ecological health status of Deeper Beel. This study is aimed at generating baseline information to aid long-term monitoring and restoration of the Deepor Beel as well as the first comprehensive assessment of a RAMSAR Site located in northeast of India.
摘要：
DeeporBeel,位于印度的阿萨姆邦，是具有野生动物保护区的国际重要湿地，并且是该州唯一的RAMSAR站点。尽管具有宝贵的生态意义，湿地面临着人为压力，导致生态健康迅速退化。2022年12月，从DeeporBeel的六个站点收集了地表水，以使用eDNA方法阐明生物群落。在采样时，原位环境参数测量一式三份。分别使用UV-Vis分光光度法和ICP-MS方法估算了溶解的营养素以及金属和准金属的浓度。研究表明，地表水中溶解的硝酸盐浓度很高。在MinION平台中使用Nanopore测序化学进行的高通量测序表明，Moraxellaceae（原核生物）和Eumetazoa（Eukaryotes）的压倒性丰度。在研究的湿地中也遇到了鲤科的丰富情况，反映了鱼类种群的生物多样性。高硝酸盐以及阐明的微生物信号对于指定DeeperBeel的生态健康状况至关重要。这项研究旨在生成基线信息，以帮助对DeeporBeel进行长期监测和恢复，并对位于印度东北部的RAMSAR站点进行首次全面评估。
