PDF(Pubmed)
Abstract：
This study establishes the serological frequency against Neospora caninum on day zero and the presence of N. caninum DNA surveyed throughout the gestation of Bubalus bubalis females in a bovine buffalo system in the central zone of the state of Veracruz, Mexico. Blood samples were taken from 11 females in 6 different sampling periods and analyzed for N. caninum antibodies detection on day zero. DNA detection by PCR was performed on all sampling periods. The gestation months of the females were recorded for five trimesters by ultrasonography, as well as births and pregnancy losses. Recorded seropositivity and positivity for agent DNA were 90.9% (95% CI 58.7-9.7) and 36.3% (95% CI 10.9-69.2), respectively, on day zero. N. caninum DNA was detected between 18.1% (95% CI 2.3-51.7) and 45.4% (95% CI 16.7-76.6) over the five trimesters of observation, with three births and three abortions recorded. The studied water buffalo population had a high presence of N. caninum antibodies; however, the detection of N. caninum DNA remained below 47% in the females. The association was only observed in the detection of DNA with pregnant females (P 0.007). Our results support the hypothesis of the resistance of water buffaloes to infection and the onset of clinical signs against infection by N. caninum even upon a high possibility of infection and reinfection described in this production system in Mexico.
摘要：
这项研究确定了在第0天针对新孢子虫的血清学频率，以及在韦拉克鲁斯州中部地区的牛水牛系统中，在整个Bubalusbubalis雌性妊娠期间所调查的N.caninumDNA的存在，墨西哥。在6个不同的采样周期中从11名雌性中采集血液样品,并在第0天分析犬奈瑟菌抗体检测。在所有采样周期进行通过PCR的DNA检测。通过超声检查记录女性的妊娠月份，为期五个月。以及出生和怀孕损失。记录的试剂DNA的血清阳性和阳性分别为90.9%（95%CI58.7-9.7）和36.3%（95%CI10.9-69.2），分别,在零日。在观察的五个三个月中，检测到的犬N.caninumDNA在18.1％（95％CI2.3-51.7）和45.4％（95％CI16.7-76.6）之间，记录了三次分娩和三次堕胎。所研究的水牛种群中存在较高的犬氏杆菌抗体；然而，在女性中，犬奈瑟菌DNA的检测保持在47%以下。仅在孕妇的DNA检测中观察到关联（P0.007）。我们的结果支持以下假设：水牛对感染的抵抗力以及即使在墨西哥该生产系统中描述的感染和再感染的可能性很高的情况下，也会出现抗N.caninum感染的临床症状。
