Abstract：
Cyantraniliprole is a highly effective diamide insecticide used to control of Laodelphax striatellus (Fallén). This study aimed to assess the insecticide resistance risk of L. striatellus and its metabolic resistance mechanisms. After 25 continuous generations of selection, the resistance of L. striatellus to cyantraniliprole increased by 17.14-fold. The realistic heritability of resistance was 0.0751. After successive rearing for five generations without exposure to insecticides, the resistance ratio for the resistant strain of L. striatellus decreased by 3.47-fold, and the average resistance decline rate per generation was 0.0266. Cyantraniliprole-resistant strains did not exhibit cross-resistance to triflumezopyrim, pymetrozine, flonicamid, sulfoxaflor, dinotefuran, clothianidin, thiamethoxam, nitenpyram, or imidacloprid. Compared to those of the sensitive strain, the 2nd, 3rd, and 4th instars, nymphal stage durations, total preoviposition period, and average generation time of the resistant strain were markedly reduced. Furthermore, the activity of cytochrome P450 monooxygenase (P450) and carboxylesterase (CarE) were markedly increased. The upregulation of CYP419A1v2 expression was most evident among the P450 genes, with a 6.10-fold increase relative to that in the sensitive strain. The CarE gene LsCarE5 was significantly upregulated by 1.94-fold compared with that in the sensitive strain. With the continuous use of cyantraniliprole, L. striatellus may develop resistance to this insecticide. This resistance may be related to the increase in metabolic enzyme activities regulated by the overexpression of P450 and CarE genes.
摘要：
Cyantraniliprole是一种高效的二酰胺杀虫剂，用于控制Laodelphas纹状体（Fallén）。本研究旨在评估纹枯病菌对杀虫剂的抗性风险及其代谢抗性机制。经过25个连续的世代选择，纹状体对蓝藻的抗性增加了17.14倍。抗性的现实遗传力为0.0751。在连续饲养五代而不接触杀虫剂后，纹枯病菌抗性菌株的抗性比下降了3.47倍，平均每代抗性下降率为0.0266。耐蓝藻的菌株对三氟美唑吡林没有表现出交叉抗性，吡虫啉,Flonicamid,sulfxaflor,dinotfuran,clothianidin,噻虫嗪,尼坦吡喃,或者吡虫啉.与敏感菌株相比，第二，3rd,第四英寸，若虫阶段持续时间，总产前期，抗性菌株的平均世代时间明显缩短。此外，细胞色素P450单加氧酶（P450）和羧酸酯酶（CarE）的活性显着增加。CYP419A1v2表达上调在P450基因中最为明显,相对于敏感菌株增加了6.10倍。与敏感菌株相比，CarE基因LsCarE5显着上调了1.94倍。随着蓝藻的不断使用，纹枯病菌可能对这种杀虫剂产生抗性。这种抗性可能与P450和CarE基因过表达调节的代谢酶活性增加有关。
